Invitrogen

Phospho-c-Raf (Ser621) Recombinant Rabbit Monoclonal Antibody (JJ085-05)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32438

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ085-05

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser621 of Human Raf1 aa 591-640
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809716

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-RAF; C-Raf proto-oncogene, serine/threonine kinase; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le

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Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf

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UniProt ID: (Human) P04049, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Human) 5894, (Mouse) 110157, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding metal ion binding protein heterodimerization activity nucleotide binding receptor signaling protein activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity response to hypoxia stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process signal transduction activation of adenylate cyclase activity heart development cell proliferation negative regulation of cell proliferation cell differentiation platelet activation thyroid gland development negative regulation of protein complex assembly positive regulation of peptidyl-serine phosphorylation ion transmembrane transport somatic stem cell population maintenance regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch wound healing regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway thymus development face development death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell motility cellular glucose homeostasis positive regulation of protein phosphorylation phosphorylation intracellular signal transduction
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