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Phospho-eIF4B (Ser422) Antibody (PA5-97393) in WB
Western blot analysis of Phospho-eIF4B (Ser422) in extracts of HeLa cells. Samples were incubated with Phospho-eIF4B (Ser422) Polyclonal antibody (Product # PA5-97393) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by EGF (100 ng/mL) for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane... View More
Product Details
PA5-97393
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S422 of human EIF4B (NP_0014082)
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809195
Product Specific Information
Immunogen sequence: TGSES; Positive Samples: HeLa
Target Information
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609
Gene Aliases: EIF-4B; EIF4B; PRO1843
UniProt ID: (Human) P23588
Entrez Gene ID: (Human) 1975
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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