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Invitrogen
Phospho-eIF4E (Ser209) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Phospho-eIF4E (Ser209) Antibody (44-528G) in ICC/IF
Immunofluorescence analysis of Phospho-eIF4E pSer209 was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho eIF4E pSer209 Rabbit Polyclonal Antibody (Product # 44-528G) at 1:250 dilution in 0.1% BSA and inc... View More
Product Details
44-528G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF4E that contains serine 209. The sequence is conserved in mouse, rat and rabbit.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533674
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705
Gene Aliases: AUTS19; CBP; EG668879; EIF-4E; EIF4E; Eif4e-ps; EIF4E1; EIF4EL1; EIF4F; If4e
UniProt ID: (Human) P06730, (Rabbit) P29338, (Mouse) P63073, (Rat) P63074
Entrez Gene ID: (Human) 1977, (Rabbit) 100009338, (Mouse) 13684, (Rat) 117045
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA poly(A) tail shortening
behavioral fear response
RNA export from nucleus
mRNA export from nucleus
translational initiation
regulation of translation
viral process
negative regulation of translation
stem cell population maintenance
lung development
negative regulation of neuron differentiation
positive regulation of mitotic cell cycle
translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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