Invitrogen

Phospho-eNOS (Ser1177) Polyclonal Antibody

3 Published Figures

11 References

View all (45) eNOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

Phospho-eNOS (Ser1177) Antibody in Western Blot (WB)

FIGURE: 1 / 3

Phospho-eNOS (Ser1177) Antibody (PA5-17917) in WB

Scientific reports 2020 - Figure 5 Effects of Serelaxin on post-GMH brain inflammation. ( A ) Representative western blot results. 72 hours after GMH significant decrease of e-NOS phosphorylation in brain of none treated compared to sham operated animals was observed ( B ). Consequently, overproduction of inflammatory factors IL-6 and TNFalpha in the brain was induced ( C , D respectively). Serelaxin reversed GMH effect on e-NOS pho... View More

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Product Details

PA5-17917

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer1177 of human eNOS

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

79 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986074

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury.

International journal of biological sciences

Ock J,Wu J,Liu FY,Fridayana FR,Niloofar L,Vo MN,Hong SS,Kang JH,Suh JK,Yin GN,Jin HR,Ryu JK

PA5-17917 was used in Immunohistochemistry (Frozen) to provide new insights into Hebp1 as a neurovascular regeneration factor and demonstrate its potential therapeutic application to various peripheral nerve injuries.

Fri Jun 23 00:00:00 EDT 2023

Artemisinin and Its Derivate Alleviate Pulmonary Hypertension and Vasoconstriction in Rodent Models.

Oxidative medicine and cellular longevity

Bao C,He Q,Wang H,Sun Y,Xu Y,Pan Y,Hu Y,Zheng S,Liang S,Luo A,Nahar T,Chen J,Tang H,Han Y

PA5-17917 was used in Western Blotting to indicate that treatment with ARS or DHA can inhibit PA vasoconstriction, PASMC proliferation and migration, and vascular remodeling, as well as improve PA endothelium-dependent relaxation, and eventually attenuate the development and progression of PH.

Tue Jun 28 00:00:00 EDT 2022

Partial Synthetic PPAR¿ Derivative Ameliorates Aorta Injury in Experimental Diabetic Rats Mediated by Activation of miR-126-5p Pi3k/AKT/PDK 1/mTOR Expression.

Pharmaceuticals (Basel, Switzerland)

Ahmed YM,Orfali R,Abdelwahab NS,Hassan HM,Rateb ME,AboulMagd AM

PA5-17917 was used in Western Blotting to reveal that HFD/STZ-induced T2D increased fasting blood glucose, ET-1, ICAM-1, E-selectin, and VIP levels, while decreasing the expression of both microRNA126-5p and p-AKT/p-Pi3k/p-PDK-1/p-mTOR phosphorylation.

Thu Sep 22 00:00:00 EDT 2022

Blood pressure-independent inhibition of Marfan aortic root widening by the angiotensin II receptor blocker valsartan.

Physiological reports

Tehrani AY,White Z,Milad N,Esfandiarei M,Seidman MA,Bernatchez P

PA5-17917 was used in Immunohistochemistry (PFA fixed) to support the concept of achieving aortic root stability with valsartan in absence of BP-lowering effects, which may help address efficacy and compliance issues with losartan-based MFS patient management.

Sat May 01 00:00:00 EDT 2021

Perfluorooctane sulfonic acid (PFOS) exposure during pregnancy increases blood pressure and impairs vascular relaxation mechanisms in the adult offspring.

Reproductive toxicology (Elmsford, N.Y.)

Dangudubiyyam SV,Mishra JS,Zhao H,Kumar S

PA5-17917 was used in Western Blot to examine whether PFOS exposure during pregnancy alters blood pressure in male and female offspring, and if this is related to sex-specific changes in vascular mechanisms.

Tue Dec 01 00:00:00 EST 2020

Neurogranin regulates eNOS function and endothelial activation.

Redox biology

Cheriyan VT,Alfaidi M,Jorgensen AN,Alam MA,Abdullah CS,Kolluru GK,Bhuiyan MS,Kevil CG,Orr AW,Nam HW

PA5-17917 was used in Western Blotting to demonstrate that neurogranin plays a crucial role in Ca2+-CaM-dependent endothelial nitric oxide synthase regulation and contributes to vascular remodelling.

Wed Jul 01 00:00:00 EDT 2020

Neurogranin regulates eNOS function and endothelial activation.

Redox biology

Cheriyan VT,Alfaidi M,Jorgensen AN,Alam MA,Abdullah CS,Kolluru GK,Bhuiyan MS,Kevil CG,Orr AW,Nam HW

PA5-17917 was used in Western Blotting to demonstrate that neurogranin plays a crucial role in Ca2+-CaM-dependent endothelial nitric oxide synthase regulation and contributes to vascular remodelling.

Wed Jul 01 00:00:00 EDT 2020

Serelaxin activates eNOS, suppresses inflammation, attenuates developmental delay and improves cognitive functions of neonatal rats after germinal matrix hemorrhage.

Scientific reports

Xu MM,Seyler L,Bäuerle T,Kalinichenko LS,Müller CP,Huttner HB,Schwab S,Manaenko A

PA5-17917 was used in Western Blotting to investigate the beneficial effects of Serelaxin on brain injury after germinal matrix hemorrhage in rats.

Fri May 15 00:00:00 EDT 2020

Serelaxin activates eNOS, suppresses inflammation, attenuates developmental delay and improves cognitive functions of neonatal rats after germinal matrix hemorrhage.

Scientific reports

Xu MM,Seyler L,Bäuerle T,Kalinichenko LS,Müller CP,Huttner HB,Schwab S,Manaenko A

PA5-17917 was used in Western Blotting to investigate the beneficial effects of Serelaxin on brain injury after germinal matrix hemorrhage in rats.

Fri May 15 00:00:00 EDT 2020

Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.

Journal of molecular and cellular cardiology

Waddingham MT,Sonobe T,Tsuchimochi H,Edgley AJ,Sukumaran V,Chen YC,Hansra SS,Schwenke DO,Umetani K,Aoyama K,Yagi N,Kelly DJ,Gaderi S,Herwig M,Kolijn D,Mügge A,Paulus WJ,Ogo T,Shirai M,Hamdani N,Pearson JT

PA5-17917 was used in Western Blotting to examine if diastolic dysfunction at the level of the cardiomyocytes precedes coronary endothelial dysfunction in prediabetes.

Sun Dec 01 00:00:00 EST 2019

Widespread Coronary Dysfunction in the Absence of HDL Receptor SR-B1 in an Ischemic Cardiomyopathy Mouse Model.

Scientific reports

Pearson JT,Yoshimoto M,Chen YC,Sultani R,Edgley AJ,Nakaoka H,Nishida M,Umetani K,Waddingham MT,Jin HL,Zhang Y,Kelly DJ,Schwenke DO,Inagaki T,Tsuchimochi H,Komuro I,Yamashita S,Shirai M

Published figure using Phospho-eNOS (Ser1177) polyclonal antibody (Product # PA5-17917) in Western Blot

Fri Dec 22 00:00:00 EST 2017

Role of aromatase in sex-specific cerebrovascular endothelial function in mice.

American journal of physiology. Heart and circulatory physiology

Zuloaga KL,Davis CM,Zhang W,Alkayed NJ

PA5-17917 was used in western blot to study gender differences in murine cerebrovascular endothelial function and the role of aromatase

Tue Apr 01 00:00:00 EDT 2014

Methylene blue modulates transendothelial migration of peripheral blood cells.

PloS one

Werner I,Guo F,Bogert NV,Stock UA,Meybohm P,Moritz A,Beiras-Fernandez A

PA5-17917 was used in western blot to study the mechanisms underlying the modulation of peripheral blood cell transendothelial migration by methylene blue

Fri Sep 19 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: cNOS; EC-NOS; ECNOS; eNOS; NOS; NOS3; NOSIII

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UniProt ID: (Pig) Q28969, (Human) P29474

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Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846

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Function(s)

nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding actin monomer binding iron ion binding protein binding tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding oxidoreductase protein modifying enzyme

Process(es)

Performance Guarantee

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Phospho-eNOS (Ser1177) Polyclonal Antibody

Phospho-eNOS (Ser1177) Antibody (PA5-17917) in WB

Product Details

PA5-17917

Western Blot (WB)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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