Bioss

Phospho-eNOS (Ser615) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

BS-13073R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser615.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: 2310065A03Rik; ECNOS; eNOS; Nos-3; NOS3

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UniProt ID: (Human) P29474, (Mouse) P70313, (Rat) O88672

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Entrez Gene ID: (Human) 4846, (Mouse) 18127, (Rat) 24600

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Function(s)
actin monomer binding nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding heme binding tetrahydrobiopterin binding arginine binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding actin binding NADPH-hemoprotein reductase activity beta-catenin binding oxidoreductase activity cadherin binding metal ion binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase protein modifying enzyme
Process(es)
angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin arginine catabolic process nitric oxide biosynthetic process mitochondrion organization nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development positive regulation of guanylate cyclase activity lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process negative regulation of extrinsic apoptotic signaling pathway via death domain receptors signal transduction response to estradiol positive regulation of apoptotic process negative regulation of smooth muscle cell proliferation cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to lipopolysaccharide response to cytokine cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
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