Invitrogen

Phospho-p27 Kip1 (Ser10) Polyclonal Antibody

4 Published Figures

12 References

View all (84) p27Kip1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

Phospho-p27 Kip1 (Ser10) Antibody in Western Blot (WB)

FIGURE: 1 / 5

Phospho-p27 Kip1 (Ser10) Antibody (34-6300) in WB

Western blot analysis of recombinant purified wild-type p27 and p27 (S10A) mutants after phosphorylation in vivo by either cyclin E-Cdk2 (lanes 1 and 2) or ERK2 (lanes 3 and 4) using Rb anti-phospho-p27 (Ser10) (Product # 34-6300) (top), Rb anti-phospho-p27 (Thr187) (Product # 71-7700) (middle), and a monoclonal antibody to p27 (bottom). Image reproduced from Rodier G, et al. EMBO J 2001.

Phospho-p27 Kip1 (Ser10) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

34-6300

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533180

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MARCKS Signaling Differentially Regulates Vascular Smooth Muscle and Endothelial Cell Proliferation through a KIS-, p27kip1- Dependent Mechanism.

PloS one

Yu D,Makkar G,Dong T,Strickland DK,Sarkar R,Monahan TS

Published figure using Phospho-p27 Kip1 (Ser10) polyclonal antibody (Product # 34-6300) in Western Blot

Fri Jun 10 00:00:00 EDT 2016

MARCKS Signaling Differentially Regulates Vascular Smooth Muscle and Endothelial Cell Proliferation through a KIS-, p27kip1- Dependent Mechanism.

PloS one

Yu D,Makkar G,Dong T,Strickland DK,Sarkar R,Monahan TS

Published figure using Phospho-p27 Kip1 (Ser10) polyclonal antibody (Product # 34-6300) in Western Blot

Fri Jun 10 00:00:00 EDT 2016

Cdk5 and its substrates, Dcx and p27kip1, regulate cytoplasmic dilation formation and nuclear elongation in migrating neurons.

Development (Cambridge, England)

Nishimura YV,Shikanai M,Hoshino M,Ohshima T,Nabeshima Y,Mizutani K,Nagata K,Nakajima K,Kawauchi T

34-6300 was used in western blot to elucidate the molecular mechanisms of dilation formation and nuclear elongation

Mon Sep 01 00:00:00 EDT 2014

Expression of CRM1 in human gliomas and its significance in p27 expression and clinical prognosis.

Neurosurgery

Shen A,Wang Y,Zhao Y,Zou L,Sun L,Cheng C

34-6300 was used in western blot to test if CRM1, Ser10-phosphorylated p27, and p27 correlated with each other, with glioma pathological stage, and with patient outcome

Wed Jul 01 00:00:00 EDT 2009

Expression of Jun activation domain-binding protein 1 and Ser10 phosphorylated p27 protein in human epithelial ovarian carcinoma.

Journal of cancer research and clinical oncology

Wang Y,Wang Y,Cheng C,Ji Y,Zhao Y,Zou L,Shen A

34-6300 was used in western blot to test if Jab1 expression is correlated with p27 protein in epithelial ovarian carcinoma

Wed Jul 01 00:00:00 EDT 2009

Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.

Molecular and cellular biology

James MK,Ray A,Leznova D,Blain SW

34-6300 was used in western blot to study if the growth state-dependent tyrosine phosphorylation of p27Kip1 determines whether or not it inhibits cdk4

Tue Jan 01 00:00:00 EST 2008

Jun activation domain-binding protein 1 negatively regulate p27 kip1 in non-Hodgkin's lymphomas.

Cancer biology & therapy

Wang Y,Fei M,Cheng C,Zhang D,Lu J,He S,Zhao Y,Wang Y,Shen A

34-6300 was used in western blot to correlate Jab1 expression and p27kip1 levels in Non-Hodgkin's Lymphomas

Sat Mar 01 00:00:00 EST 2008

Endothelin is a dose-dependent trophic factor and a mitogen in small arteries in vivo.

Cardiovascular research

Dao HH,Bouvet C,Moreau S,Beaucage P,Larivière R,Servant MJ,de Champlain J,Moreau P

34-6300 was used in western blot to discuss the contribution of endothelin to vascular remodeling

Sat Jul 01 00:00:00 EDT 2006

Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells.

The Journal of biological chemistry

Seo DW,Li H,Qu CK,Oh J,Kim YS,Diaz T,Wei B,Han JW,Stetler-Stevenson WG

Fri Feb 10 00:00:00 EST 2006

Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells.

The Journal of biological chemistry

Seo DW,Li H,Qu CK,Oh J,Kim YS,Diaz T,Wei B,Han JW,Stetler-Stevenson WG

Fri Feb 10 00:00:00 EST 2006

Deletion of Cdkn1b ameliorates hyperglycemia by maintaining compensatory hyperinsulinemia in diabetic mice.

Nature medicine

Uchida T,Nakamura T,Hashimoto N,Matsuda T,Kotani K,Sakaue H,Kido Y,Hayashi Y,Nakayama KI,White MF,Kasuga M

Tue Feb 01 00:00:00 EST 2005

The cyclin-dependent kinase inhibitor p27Kip1 is stabilized in G(0) by Mirk/dyrk1B kinase.

The Journal of biological chemistry

Deng X,Mercer SE,Shah S,Ewton DZ,Friedman E

Fri May 21 00:00:00 EDT 2004

The role of cyclin-dependent kinase inhibitor p27Kip1 in anti-HER2 antibody-induced G1 cell cycle arrest and tumor growth inhibition.

The Journal of biological chemistry

Le XF,Claret FX,Lammayot A,Tian L,Deshpande D,LaPushin R,Tari AM,Bast RC

Fri Jun 27 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; P27 CDKN1B; p27(KIP1); p27Kip1

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Gene Aliases: AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

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UniProt ID: (Human) P46527, (Mouse) P46414

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Entrez Gene ID: (Human) 1027, (Mouse) 12576, (Rat) 83571

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity transforming growth factor beta receptor, cytoplasmic mediator activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process protein phosphatase binding Hsp70 protein binding protein complex binding chaperone binding protein kinase inhibitor activity kinase activity kinase inhibitor

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle response to hypoxia potassium ion transport activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Notch signaling pathway sensory perception of sound positive regulation of cell proliferation negative regulation of cell proliferation response to glucose positive regulation of cell death negative regulation of cell growth positive regulation of microtubule polymerization response to estradiol negative regulation of kinase activity negative regulation of phosphorylation response to drug negative regulation of apoptotic process response to amino acid response to peptide hormone positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle response to cadmium ion autophagic cell death inner ear development negative regulation of cellular component movement negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to antibiotic cellular response to lithium ion cellular response to organic cyclic compound mitotic cell cycle arrest negative regulation of vascular smooth muscle cell proliferation cell cycle cell death regulation of cell proliferation negative regulation of epithelial cell proliferation response to organic cyclic compound phosphorylation

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Phospho-p27 Kip1 (Ser10) Polyclonal Antibody

Phospho-p27 Kip1 (Ser10) Antibody (34-6300) in WB

Product Details

34-6300

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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