Invitrogen

Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures
13 References
View all (67) p38 MAPK alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

36-8500

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 11 publications 11 publications

Immunohistochemistry (IHC)

1:250
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:250
-

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to a region surrounding the phosphorylated Thr180/Tyr182 residues of p38 MAP kinase.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533281

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primates and zebrafish based on 100% sequence homology.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; EC 2.7.11.24; ERK1; MAP 14; MAP kinase 14; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK1 antibody; MAX-interacting protein 2; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a

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Gene Aliases: CSBP; CSBP1; CSBP2; CSPB1; EXIP; MAPK14; MXI2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A

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UniProt ID: (Human) Q16539

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Entrez Gene ID: (Human) 1432

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Function(s)
protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity protein binding ATP binding protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding non-receptor serine/threonine protein kinase
Process(es)
DNA damage checkpoint activation of MAPK activity cell morphogenesis cartilage condensation angiogenesis placenta development chondrocyte differentiation glucose metabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process movement of cell or subcellular component chemotaxis signal transduction cell surface receptor signaling pathway transmembrane receptor protein serine/threonine kinase signaling pathway Ras protein signal transduction skeletal muscle tissue development response to glucose positive regulation of gene expression positive regulation of myotube differentiation myoblast differentiation involved in skeletal muscle regeneration peptidyl-serine phosphorylation fatty acid oxidation osteoclast differentiation positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide intracellular signal transduction cellular response to vascular endothelial growth factor stimulus response to muscle stretch p38MAPK cascade positive regulation of protein import into nucleus signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation positive regulation of muscle cell differentiation positive regulation of cardiac muscle cell proliferation 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation stress-induced premature senescence cellular response to virus regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion regulation of signal transduction by p53 class mediator positive regulation of reactive oxygen species metabolic process positive regulation of interleukin-12 secretion
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