Invitrogen

Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (S.417.1)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure
3 References
View all (67) p38 MAPK alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15182

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:25
-
Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Pig, Rat, Yeast

Published species

Human, Mouse, Sheep

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

S.417.1

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr180/Tyr182 of human p38 MAPK
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986952

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CG7393-PA; CRK1; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 2.7.11.24; ERK1; LD24658p; MAP 14; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAP kinase p38b; MAPK 14; MAPK1 antibody; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38b; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38 mitogen-activated protein kinase; p38alpha Exip; p38b-PA; reactive kinase; RP1-179N16.5; SAPK2a; stress-activated p38b MAP kinase; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38

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Gene Aliases: 186F5S; anon-sts23; BG:DS00797.3; CG7393; CRK1; CSBP; CSBP1; CSBP2; CSPB1; D-p38; D-p38 MAPK; D-p38b; Dm p38b; Dmel\CG7393; Dmel_CG7393; Dmp38b; Dp38; Dp38b; ESTS:186F5S; EXIP; Hog; MAPK; MAPK14; Mpk34C; MXI2; P38; p38 beta; p38 MAPK; p38-alpha; p38a; p38ALPHA; p38B; p38beta; p38Hog; p38Kb; p38MAPK; PRKM14; PRKM15; RK; SAPK2A

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UniProt ID: (Human) Q16539, (Mouse) P47811, (Fruit fly) O61443

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Entrez Gene ID: (Human) 1432, (Pig) 100156630, (Rat) 81649, (Mouse) 26416, (Fruit fly) 34780

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Function(s)
protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity protein binding ATP binding protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding protein kinase activity kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
DNA damage checkpoint activation of MAPK activity cell morphogenesis cartilage condensation angiogenesis placenta development chondrocyte differentiation glucose metabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process movement of cell or subcellular component chemotaxis signal transduction cell surface receptor signaling pathway transmembrane receptor protein serine/threonine kinase signaling pathway Ras protein signal transduction skeletal muscle tissue development response to glucose positive regulation of gene expression positive regulation of myotube differentiation myoblast differentiation involved in skeletal muscle regeneration peptidyl-serine phosphorylation fatty acid oxidation osteoclast differentiation positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide intracellular signal transduction cellular response to vascular endothelial growth factor stimulus response to muscle stretch p38MAPK cascade positive regulation of protein import into nucleus signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation positive regulation of muscle cell differentiation positive regulation of cardiac muscle cell proliferation 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation stress-induced premature senescence cellular response to virus regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion regulation of signal transduction by p53 class mediator positive regulation of reactive oxygen species metabolic process positive regulation of interleukin-12 secretion MAPK cascade regulation of transcription, DNA-templated protein phosphorylation cellular response to DNA damage stimulus response to lipopolysaccharide stress-activated MAPK cascade phosphorylation positive regulation of protein phosphorylation heart morphogenesis immune response imaginal disc-derived wing morphogenesis circadian rhythm determination of adult lifespan response to heat response to bacterium response to salt stress regulation of gene expression regulation of BMP signaling pathway positive regulation of actin filament polymerization cellular response to reactive oxygen species negative regulation of hippo signaling paracrine signaling positive regulation of multicellular organism growth response to hydrogen peroxide response to starvation defense response to bacterium regulation of innate immune response positive regulation of cell size regulation of adult chitin-containing cuticle pigmentation defense response to fungus cellular response to arsenic-containing substance cellular response to cadmium ion regulation of cellular response to oxidative stress regulation of terminal button organization
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