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Proteintech
Phospho-p70 (S6K) (Thr229) Recombinant Rabbit Monoclonal Antibody (2G19)
FIGURE: 1 / 1
Phospho-p70 (S6K) (Thr229) Antibody (81592-1-RR) in WB
Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 81592-1-RR (Phosoho-p70(S6K) (Thr229) antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with 60004-1-Ig (GAPDH antibody) as loading control.
Product Details
81592-1-RR
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2G19
Immunogen
Peptide
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A
Gene Aliases: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A
UniProt ID: (Human) P23443
Entrez Gene ID: (Human) 6198
G1/S transition of mitotic cell cycle
behavioral fear response
skeletal muscle contraction
protein phosphorylation
apoptotic process
signal transduction
germ cell development
aging
response to nutrient
long-term memory
response to heat
response to wounding
response to mechanical stimulus
response to toxic substance
response to glucose
skeletal muscle atrophy
response to electrical stimulus involved in regulation of muscle adaptation
positive regulation of smooth muscle cell migration
cell migration
TOR signaling
response to lipopolysaccharide
response to insulin
cellular response to hormone stimulus
response to testosterone
response to glucagon
response to tumor necrosis factor
response to drug
negative regulation of apoptotic process
response to leucine
protein kinase B signaling
response to ethanol
positive regulation of translation
positive regulation of mitotic cell cycle
positive regulation of translational initiation
regulation of glucose import
negative regulation of insulin receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of skeletal muscle tissue growth
positive regulation of smooth muscle cell proliferation
response to glucocorticoid
cellular response to growth factor stimulus
cellular response to organic cyclic compound
negative regulation of extrinsic apoptotic signaling pathway
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