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Phosphothreonine Antibody (500-301-263) in ELISA
ELISA results of Mab anti-phosphothreonine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1µg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate were used for detection.
Product Details
500-301-263
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
18F6
Immunogen
phosphothreonine conjugated KLH
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This product is clarified ascites produced in balb/c mice using clone 18F6. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
Target Information
Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C4H10NO6P; O-phospho-L-threonine; Phospho-threonine; pThr; Threonine
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