Invitrogen

Phosphothreonine Monoclonal Antibody (PT-5H5)

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Published Figures (7)

Phosphothreonine Antibody in Western Blot (WB)

FIGURE: 1 / 7

Phosphothreonine Antibody (13-9200) in WB

Cellular and molecular gastroenterology and hepatology 2019 - Figure 13 Downstream targets and upstream activators of STK25. ( A ) Liver protein lysates from high-fat-fed mice treated with GalNAc- Stk25 ASO vs GalNAc-control ASO (12.5 mg/kg/wk) were analyzed by Western blot using antibodies specific for total ACC or phospho-ACC (Ser79), with pan-actin used as a loading control. ( B and C ) Liver lysates were collected from male C57BL/6J mice ( B ) fed a normal chow di... View More

Phosphothreonine Antibody in Immunoprecipitation (IP)

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Product Details

13-9200

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 9 publications 9 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

PT-5H5

Immunogen

Phosphothreonine containing proteins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533043

Target Information

Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FAK regulates actin polymerization during sperm capacitation via the ERK2/GEF-H1/RhoA signaling pathway.

Journal of cell science

Salgado-Lucio ML,Ramírez-Ramírez D,Jorge-Cruz CY,Roa-Espitia AL,Hernández-González EO

13-9200 was used in Immunoprecipitation to investigate if FAK is involved in ERK2 activation and if an inhibition of FAK results in a significant decrease of ERK2 activation.

Tue Apr 28 00:00:00 EDT 2020

FAK regulates actin polymerization during sperm capacitation via the ERK2/GEF-H1/RhoA signaling pathway.

Journal of cell science

Salgado-Lucio ML,Ramírez-Ramírez D,Jorge-Cruz CY,Roa-Espitia AL,Hernández-González EO

13-9200 was used in Immunoprecipitation to investigate if FAK is involved in ERK2 activation and if an inhibition of FAK results in a significant decrease of ERK2 activation.

Tue Apr 28 00:00:00 EDT 2020

Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease.

Cellular and molecular gastroenterology and hepatology

Cansby E,Nuñez-Durán E,Magnusson E,Amrutkar M,Booten SL,Kulkarni NM,Svensson LT,Borén J,Marschall HU,Aghajan M,Mahlapuu M

13-9200 was used in Western Blotting to provide in vivo nonclinical proof-of-principle for the metabolic benefit of liver-specific inhibition of STK25 in the context of obesity and warrants future investigations to address the therapeutic potential of GalNAc-Stk25 ASO in the prevention and treatment of NAFLD.

Tue Jun 04 00:00:00 EDT 2019

Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability.

Oncogene

Kharat SS,Tripathi V,Damodaran AP,Priyadarshini R,Chandra S,Tikoo S,Nandhakumar R,Srivastava V,Priya S,Hussain M,Kaur S,Fishman JB,Sengupta S

13-9200 was used in western blot to show that that BLM undergoes K48-linked ubiquitylation and subsequent degradation during mitosis via Fbw7alpha

Thu Feb 25 00:00:00 EST 2016

Protein phosphorylation during Plasmodium berghei gametogenesis.

Experimental parasitology

Alonso-Morales A,González-López L,Cázares-Raga FE,Cortés-Martínez L,Torres-Monzón JA,Gallegos-Pérez JL,Rodríguez MH,James AA,Hernández-Hernández Fde L

13-9200 was used in western blot to investigate protein phosphorylation during Plasmodium berghei gametogenesis and its role

Tue Sep 01 00:00:00 EDT 2015

Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wang X,Cahill ME,Werner CT,Christoffel DJ,Golden SA,Xie Z,Loweth JA,Marinelli M,Russo SJ,Penzes P,Wolf ME

13-9200 was used in western blot to study the role of kalirin-7 in the mechanism by which cocaine-induced AMPAR and spine upregulation lead to incentive sensitization.

Wed Jul 03 00:00:00 EDT 2013

Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wang X,Cahill ME,Werner CT,Christoffel DJ,Golden SA,Xie Z,Loweth JA,Marinelli M,Russo SJ,Penzes P,Wolf ME

13-9200 was used in western blot to study the role of kalirin-7 in the mechanism by which cocaine-induced AMPAR and spine upregulation lead to incentive sensitization.

Wed Jul 03 00:00:00 EDT 2013

Investigation of the lateral light-induced migration of photosystem II light-harvesting proteins by nano-high performance liquid chromatography electrospray ionization mass spectrometry.

The Journal of biological chemistry

Timperio AM,Zolla L

13-9200 was used in western blot to assess photosystem II light-harvesting proteins by an investigation of the lateral light-induced migration by nano-high performance liquid chromatography electrospray ionization mass spectrometry

Fri Aug 12 00:00:00 EDT 2005

Constitutive induction of p-Erk1/2 accompanied by reduced activities of protein phosphatases 1 and 2A and MKP3 due to reactive oxygen species during cellular senescence.

The Journal of biological chemistry

Kim HS,Song MC,Kwak IH,Park TJ,Lim IK

Published figure using Phosphothreonine monoclonal antibody (Product # 13-9200) in Western Blot

Fri Sep 26 00:00:00 EDT 2003

A novel homocysteine-responsive gene, smap8, modulates mitogenesis in rat vascular smooth muscle cells.

European journal of biochemistry

Nishimoto S,Tawara J,Toyoda H,Kitamura K,Komurasaki T

13-9200 was used in western blot to characterize modulation of mitogenesis in rat vascular smooth muscle cells by a novel homocysteine-responsive gene, smap8

Sun Jun 01 00:00:00 EDT 2003

Kinase suppressor of Ras signals through Thr269 of c-Raf-1.

The Journal of biological chemistry

Xing HR,Kolesnick R

Published figure using Phosphothreonine monoclonal antibody (Product # 13-9200) in Western Blot

Fri Mar 30 00:00:00 EST 2001

Bioinformatics

Protein Aliases: C4H10NO6P; O-phospho-L-threonine; Phospho-threonine; pThr; Threonine

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phosphothreonine Monoclonal Antibody (PT-5H5)

Phosphothreonine Antibody (13-9200) in WB

Product Details

13-9200

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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