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PlGF-1 Antibody (500-P226BT-25UG) in ELISA
Sandwich ELISA: To detect hPlGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of PlGF-1 Polyclonal Antibody, Biotin (Product # 500-P226BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech PlGF-1 Polyclonal Antibody (Product # 500-P226-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPlGF.
Product Details
500-P226BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 29.7 kDa Recombinant Human PlGF-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929910
Product Specific Information
AA Sequence of recombinant protein: MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PlGF-1. Anti-Human PlGF-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hPlGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PlGF (500-P226) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPlGF.
Western Blot: To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPlGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P226BT-1MG will be provided as 2 x 500 µg
Target Information
PlGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-1 is expressed in placental tissues, the colon, and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Placenta Growth Factor; Placenta Growth Factor-1; placental growth factor, vascular endothelial growth factor-related protein; PlGF
Gene Aliases: D12S1900; PGF; PGFL; PIGF; PLGF; PlGF-2; SHGC-10760
UniProt ID: (Human) P49763
Entrez Gene ID: (Human) 5228
branching involved in ureteric bud morphogenesis
response to hypoxia
positive regulation of endothelial cell proliferation
sprouting angiogenesis
signal transduction
cell-cell signaling
female pregnancy
positive regulation of cell proliferation
cell differentiation
organ regeneration
cellular response to hormone stimulus
response to drug
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
positive regulation of cell division
regulation of morphogenesis of a branching structure
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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