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Invitrogen
Podocin Recombinant Rabbit Monoclonal Antibody (2O10G3)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Podocin Antibody (MA5-42776) in WB
Western blot was performed using Podocin Recombinant Rabbit Monoclonal Antibody (2O10G3) (Product # MA5-42776) and a ~45 kDa band corresponding to Podocin was observed in Mouse Kidney tissue. Whole cell extracts (30 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Pr... View More
Product Details
MA5-42776
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2O10G3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 284-383 of human NPHS2 (Q9NP85).
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911917
Product Specific Information
Positive test controls include: HeLa, HT-29, 293T.
Immunogen sequence: AQRQAKVRMI AAEAEKAASE SLRMAAEILS GTPAAVQLRY LHTLQSLSTE KPSTVVLPLP FDLLNCLSSP SNRTQGSLPF PSPSKPVEPL NPKKKDSPML
Target Information
The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: nephrosis 2 homolog, podocin; nephrosis 2 idiopathic steroid-resistant (podocin); nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2 podocin; Podocin
Gene Aliases: NPHS2; PDCN; podocin; SRN1
UniProt ID: (Human) Q9NP85, (Rat) Q8K4G9
Entrez Gene ID: (Human) 7827, (Rat) 170672
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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