Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Podoplanin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Podoplanin Antibody (PA5-37285) in WB
Western blot was performed using Anti-Podoplanin Polyclonal Antibody(Product # PA5-37285) and a 50 kDa band corresponding to Podoplanin was observed across tissues and cell lines tested. Whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse mammary gland (Lane 2), Rat Liver (Lane 3), Rat mammary gland (Lane 4), NTERA-2 cl.D1 (Lane 5), U-2 OS (Lane 5), HeLa (Lane 6), A549 (Lane 7), BeWo (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitro... View More
Product Details
PA5-37285
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to residues in human Podoplanin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2553996
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 37 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Aggrus; CTA-520D8.1; E11; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; Glycoprotein 36; Glycoprotein 38; glycoprotein, 36-KD; Gp36; Gp38; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; PDPN; Podoplanin; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RP23-348F1.2; RTI140; T1-alpha; T1A; Transmembrane glycoprotein E11; Type I cell 40 kDa protein
Gene Aliases: AGGRUS; E11; GP36; Gp38; GP40; HT1A-1; OTS-8; OTS8; PA2.26; PDPN; PSEC0003; PSEC0025; RANDAM-2; RTI40; T1-alpha; T1A; T1A-2; T1A2; T1alpha; TI1A
UniProt ID: (Human) Q86YL7, (Rat) Q64294, (Mouse) Q62011
Entrez Gene ID: (Human) 10630, (Rat) 54320, (Mouse) 14726
cell morphogenesis
lymphangiogenesis
prostaglandin metabolic process
water transport
amino acid transport
signal transduction
cell proliferation
regulation of cell shape
folic acid transport
single organismal cell-cell adhesion
platelet activation
lung development
lung alveolus development
cell motility
positive regulation of cellular component movement
regulation of G1/S transition of mitotic cell cycle
inflammatory response
cell adhesion
nervous system development
positive regulation of platelet activation
positive regulation of cell migration
tube morphogenesis
response to hyperoxia
movement of cell or subcellular component
multicellular organism development
visceral serous pericardium development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support