Invitrogen

Podoplanin Monoclonal Antibody (NZ-1.3), APC, eBioscience™

7 Published Figures

13 References

View all (70) Podoplanin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

Podoplanin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

Podoplanin Antibody (17-9381-42) in Flow

Staining of U87 cell line with Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (blue histogram) or Anti-Human Podoplanin APC (purple histogram). Total viable cells were used for analysis.

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Product Details

17-9381-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NZ-1.3

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10801951

Product Specific Information

Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis. This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma. Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex. Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2).

This antibody has been reported to block the interaction between Podoplanin and CLEC-2.

Applications Reported: This NZ-1.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NZ-1.3 antibody has been pre-titrated and tested by flow cytometric analysis of U87 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Denervation during mandibular distraction osteogenesis results in impaired bone formation.

Scientific reports

Tevlin R,Griffin M,Chen K,Januszyk M,Guardino N,Spielman A,Walters S,Gold GE,Chan CKF,Gurtner GC,Wan DC,Longaker MT

17-9381-42 was used in Flow cytometry/Cell sorting to show that the osteogenic potential of DO-Den SSCs was increased in comparison to control untreated DO-Den SSCs, modeling the superior osteogenic potential of DO-Inn SSCs.

Mon Feb 06 00:00:00 EST 2023

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Published figure using Podoplanin monoclonal antibody (Product # 17-9381-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

iPSC-derived mesenchymal cells that support alveolar organoid development.

Cell reports methods

Tamai K,Sakai K,Yamaki H,Moriguchi K,Igura K,Maehana S,Suezawa T,Takehara K,Hagiwara M,Hirai T,Gotoh S

17-9381-42 was used in Flow cytometry/Cell sorting to demonstrated that the total iPSC-derived alveolar organoids were useful for characterizing responses to the influenza A (H1N1) virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, demonstrating their utility for disease modeling.

Mon Oct 24 00:00:00 EDT 2022

Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses.

Cell research

He J,Yan J,Wang J,Zhao L,Xin Q,Zeng Y,Sun Y,Zhang H,Bai Z,Li Z,Ni Y,Gong Y,Li Y,He H,Bian Z,Lan Y,Ma C,Bian L,Zhu H,Liu B,Yue R

Published figure using Podoplanin monoclonal antibody (Product # 17-9381-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration.

Cell research

Liang C,Liu Z,Song M,Li W,Wu Z,Wang Z,Wang Q,Wang S,Yan K,Sun L,Hishida T,Cai Y,Belmonte JCI,Guillen P,Chan P,Zhou Q,Zhang W,Qu J,Liu GH

17-9381 was used in Flow cytometry/Cell sorting to identify a transcription factor-independent role of CLOCK, a core component of the molecular circadian clock machinery, in counteracting human mesenchymal stem cell (hMSC) decay.

Mon Feb 01 00:00:00 EST 2021

Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis.

PLoS pathogens

Chen W,Ding Y,Liu D,Lu Z,Wang Y,Yuan Y

Published figure using Podoplanin monoclonal antibody (Product # 17-9381-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition.

Stem cells (Dayton, Ohio)

Kanagaki S,Ikeo S,Suezawa T,Yamamoto Y,Seki M,Hirai T,Hagiwara M,Suzuki Y,Gotoh S

17-9381 was used in Flow cytometry/Cell sorting to define iAT1 cells and identify FD-AOs as a useful model for investigating the mechanism underlying human AT1 cell differentiation from AT2 cells in vitro.

Mon Feb 01 00:00:00 EST 2021

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition.

Stem cells (Dayton, Ohio)

Kanagaki S,Ikeo S,Suezawa T,Yamamoto Y,Seki M,Hirai T,Hagiwara M,Suzuki Y,Gotoh S

Mon Feb 01 00:00:00 EST 2021

Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer.

International journal of molecular sciences

Wilkus K,Brodaczewska K,Kajdasz A,Kieda C

Published figure using Podoplanin monoclonal antibody (Product # 17-9381-42) in Flow Cytometry

Tue Aug 17 00:00:00 EDT 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

Published figure using Podoplanin monoclonal antibody (Product # 17-9381-42) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer.

Protein & cell

Bi S,Liu Z,Wu Z,Wang Z,Liu X,Wang S,Ren J,Yao Y,Zhang W,Song M,Liu GH,Qu J

17-9381 was used in Flow cytometry/Cell sorting to investigate if SIRT7 expression declines during human mesenchymal stem cell (hMSC) aging and if SIRT7 deficiency accelerates senescence.

Wed Jul 01 00:00:00 EDT 2020

Geriatric fragility fractures are associated with a human skeletal stem cell defect.

Aging cell

Ambrosi TH,Goodnough LH,Steininger HM,Hoover MY,Kim E,Koepke LS,Marecic O,Zhao L,Seita J,Bishop JA,Gardner MJ,Chan CKF

17-9381 was used in Flow cytometry/Cell sorting to study the role human skeletal stem cells in driving impaired healing.

Wed Jul 01 00:00:00 EDT 2020

Delayed Union of a Diaphyseal Forearm Fracture Associated With Impaired Osteogenic Differentiation of Prospectively Isolated Human Skeletal Stem Cells.

JBMR plus

Goodnough LH,Ambrosi TH,Steininger H,DeBaun MR,Abrams GD,McAdams TR,Gardner MJ,Chan CK,Bishop JA

17-9381-41 was used in Flow Cytometry to report a case in which functional in vitro readouts of skeletal stem cells(SSCs) derived from human fracture tissue at time of injury predicted a poor fracture healing outcome.

Thu Oct 01 00:00:00 EDT 2020

Delayed Union of a Diaphyseal Forearm Fracture Associated With Impaired Osteogenic Differentiation of Prospectively Isolated Human Skeletal Stem Cells.

JBMR plus

Goodnough LH,Ambrosi TH,Steininger H,DeBaun MR,Abrams GD,McAdams TR,Gardner MJ,Chan CK,Bishop JA

Thu Oct 01 00:00:00 EDT 2020

Identification of the Human Skeletal Stem Cell.

Cell

Chan CKF,Gulati GS,Sinha R,Tompkins JV,Lopez M,Carter AC,Ransom RC,Reinisch A,Wearda T,Murphy M,Brewer RE,Koepke LS,Marecic O,Manjunath A,Seo EY,Leavitt T,Lu WJ,Nguyen A,Conley SD,Salhotra A,Ambrosi TH,Borrelli MR,Siebel T,Chan K,Schallmoser K,Seita J,Sahoo D,Goodnough H,Bishop J,Gardner M,Majeti R,Wan DC,Goodman S,Weissman IL,Chang HY,Longaker MT

17-9381-42 was used in Flow cytometry/Cell sorting to combine gene expression and epigenetic data of mouse skeletal stem cells (mSSCs) and hSSCs to identify evolutionarily conserved and divergent pathways driving SSC-mediated skeletogenesis.

Thu Sep 20 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Aggrus; CTA-520D8.1; Glycoprotein 36; glycoprotein, 36-KD; Gp36; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; PDPN; Podoplanin; RP23-348F1.2; T1-alpha; T1A

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Gene Aliases: AGGRUS; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; PDPN; PSEC0003; PSEC0025; T1A; T1A-2; T1A2; TI1A

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UniProt ID: (Human) Q86YL7

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Entrez Gene ID: (Human) 10630

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Function(s)

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

Process(es)

cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport signal transduction cell proliferation regulation of cell shape folic acid transport single organismal cell-cell adhesion platelet activation lung development lung alveolus development cell motility positive regulation of cellular component movement regulation of G1/S transition of mitotic cell cycle

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Podoplanin Monoclonal Antibody (NZ-1.3), APC, eBioscience™

Podoplanin Antibody (17-9381-42) in Flow

Product Details

17-9381-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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