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Product Details
PA1-750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Sheep
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: G(90) Q G G G T H N Q W N K P(102) G G C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2172277
Product Specific Information
PA1-750 detects prion protein (PrP) from sheep tissues.
PA1-750 has been successfully used in Western blot, immunoprecipitation and immunohistochemistry procedures. By Western blot, this antibody detects a 33-35 kDa protein representing PrPC and a 27-30 kDa protein representing PrPSc from sheep normal and infected brains. Immunohistochemical staining of PrP in formalin-fixed infected sheep brain with PA1-750 results in intense staining of PrP plaques which are known to exhibit an abnormally high concentration of PrP.
PA1-750 immunizing peptide corresponds to amino acid residues 90-102 from hamster PrP. PA1-750 immunizing peptide (Cat. # PEP-034) is available for use in neutralization and control experiments.
Target Information
Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BSE; CD230; Major prion protein; MGC26679; PK resistant core; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); PrP; PrPc; TSE
Gene Aliases: PRNP; PRP; PRPC; SIP
UniProt ID: (Sheep) P23907
Entrez Gene ID: (Sheep) 493887
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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