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Product Details
MA1-82828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Sheep
Published species
Bovine,
Rabbit
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2G11
Immunogen
Synthetic peptide corresponding to 146-R(154)R(171)-182 of ovine PrP
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2237760
Product Specific Information
This antibody recognizes the scrapie-associated protease-resistant isoform (PrPsc) of ovine prion protein (PrP). The prion protein is encoded by the PrP gene and is constitutively expressed as the normal cellular isoform PrPc by many cell types. Clone 2G11 specifically recognizes the R151-R159 sequence and does not recognize the non-pathogenic PrPc form.
Pre-treat samples with trypsin at 37ºC for 5 minutes followed by heat-mediated antigen retrieval using 10mM citrate buffer (pH6.0), followed by treatment of tissue sections in 98% formic acid for 30 minutes for optimal results.
Target Information
Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BSE; CD230; Major prion protein; MGC26679; PK resistant core; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); PrP; PrPc; TSE
Gene Aliases: PRNP; PRP; PRPC; SIP
UniProt ID: (Sheep) P23907
Entrez Gene ID: (Sheep) 493887
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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