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PrP Antibody (MA5-47728) in WB
Western blot of PrP that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 minutes (lanes 6 & 8). The blots were probed with PrP monoclonal antibody (Product # MA5-47728) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser43) (lanes 3, 4, 7, & 8).
Product Details
MA5-47728
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3F4
Immunogen
Clone (3F4) was generated from a synthetic peptide taken from amino acids in the central region of human prion protein. This sequence is conserved in feline and hamster, but is less conserved in rat and mouse Prion protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942709
Product Specific Information
The antibody detects endogenous prion protein in human PC3 and A431 cell lines. In addition, the antibody strongly detects human recombinant Prion protein, and weakly detects bovine recombinant Prion protein.
Target Information
Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alternative prion protein; AltPrP; ASCR; BSE; CD230; CD230 antigen; Major prion protein; MGC26679; prion-related protein; PrP; PrP27-30; PrP33-35C; TSE
Gene Aliases: ALTPRP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PRNP; PRP; PrP27-30; PrP33-35C; PrPc
UniProt ID: (Human) F7VJQ1
Entrez Gene ID: (Human) 5621
negative regulation of protein phosphorylation
cellular copper ion homeostasis
response to oxidative stress
cell cycle arrest
learning or memory
metabolic process
negative regulation of interferon-gamma production
negative regulation of interleukin-17 production
negative regulation of interleukin-2 production
regulation of protein localization
cellular response to drug
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of activated T cell proliferation
response to cadmium ion
negative regulation of T cell receptor signaling pathway
protein homooligomerization
negative regulation of calcineurin-NFAT signaling cascade
cellular response to copper ion
regulation of potassium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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