Invitrogen

Presenilin 1 Monoclonal Antibody (APS 11)

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Protocols
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Product Details

MA1-751

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

0.75 µg/mL
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

4.5 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

APS 11

Immunogen

Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325074

Product Specific Information

MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.

MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites.

The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

Target Information

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alzheimer Disease 3; Minilin; Presenilin-1; presenilin1; Protein S182; PS-1; S182 protein; Senilin 1

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Gene Aliases: AD3; Ad3h; FAD; PS-1; PS1; PSEN1; PSNL1; S182

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UniProt ID: (Human) P49768, (Rat) P97887

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Entrez Gene ID: (Human) 5663, (Rat) 29192, (Mouse) 19164

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Function(s)
endopeptidase activity aspartic-type endopeptidase activity calcium channel activity protein binding beta-catenin binding PDZ domain binding cadherin binding peptidase activity hydrolase activity aspartic endopeptidase activity, intramembrane cleaving aspartic protease
Process(es)
autophagosome assembly negative regulation of transcription from RNA polymerase II promoter activation of MAPKK activity blood vessel development cell fate specification somitogenesis neuron migration positive regulation of receptor recycling heart looping hematopoietic progenitor cell differentiation T cell activation involved in immune response neural retina development protein glycosylation membrane protein ectodomain proteolysis mitochondrial transport cellular response to DNA damage stimulus response to oxidative stress negative regulation of epidermal growth factor-activated receptor activity Notch signaling pathway Notch receptor processing memory post-embryonic development protein transport L-glutamate transport choline transport synaptic vesicle targeting single organismal cell-cell adhesion protein processing cerebral cortex cell migration Cajal-Retzius cell differentiation dorsal/ventral neural tube patterning embryonic limb morphogenesis positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis beta-amyloid formation intracellular signal transduction regulation of phosphorylation amyloid precursor protein catabolic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of catalytic activity regulation of protein binding positive regulation of MAP kinase activity negative regulation of neuron apoptotic process skin morphogenesis positive regulation of transcription, DNA-templated regulation of synaptic plasticity thymus development neuron development skeletal system morphogenesis brain morphogenesis epithelial cell proliferation negative regulation of axonogenesis positive regulation of coagulation T cell receptor signaling pathway neuron apoptotic process negative regulation of ubiquitin-protein transferase activity smooth endoplasmic reticulum calcium ion homeostasis regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway regulation of resting membrane potential positive regulation of dendritic spine development calcium ion transmembrane transport negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway negative regulation of protein phosphorylation positive regulation of protein phosphorylation myeloid leukocyte differentiation negative regulation of protein kinase activity proteolysis calcium ion transport cellular calcium ion homeostasis autophagy regulation of epidermal growth factor-activated receptor activity brain development heart development learning or memory cerebral cortex development neurogenesis neuron differentiation forebrain development segmentation locomotion eye photoreceptor cell development cellular protein metabolic process positive regulation of protein kinase activity beta-amyloid metabolic process protein maturation apoptotic process cell adhesion embryo development anagen
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