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Invitrogen
Progesterone Receptor Monoclonal Antibody (PgR636)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Progesterone Receptor Antibody (MA5-12581) in ICC/IF
Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse mono... View More
Product Details
MA5-12581
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
PgR636
Immunogen
Formalin fixed recombinant full length A-form of human progesterone receptor
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_10979638
Product Specific Information
MA5-12581 targets Progesterone Receptor in IHC (P) applications and shows reactivity with Human samples.
The MA5-12581 immunogen is formalin fixed recombinant full length A-form of human progesterone receptor.
MA5-12581 detects A and B forms of progesterone receptor from human and mouse in western blot, immunofluorescence and immunohistochemistry.
Target Information
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor
Gene Aliases: NR3C3; PGR; PR
UniProt ID: (Human) P06401
Entrez Gene ID: (Human) 5241
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
steroid hormone receptor activity
steroid binding
protein binding
zinc ion binding
enzyme binding
ATPase binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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