Invitrogen

Progesterone Receptor Monoclonal Antibody (alpha PR-22)

Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)

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Progesterone Receptor Antibody (MA1-412) in ICC/IF

Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with or without Progesterone Receptor mouse monoclonal antibody (Product # MA1-412), at a dilution of 1:100 for 1 hour ... View More

Progesterone Receptor Antibody in Western Blot (WB)

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Product Details

MA1-412

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 7 publications 7 publications

ChIP assay (ChIP)

View 2 publications 2 publications

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Chicken, Human, Reptile

Published species

Chicken, Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

alpha PR-22

Immunogen

Progesterone receptor purified from chick oviduct cytosol.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2164317

Product Specific Information

MA1-412 detects the A and B forms of progesterone receptor from chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

MA1-412 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 78 kDa protein representing the A form and a 110 kDa protein representing the B form of PR. The proportion of receptor bound by this product is significantly greater than catalog number MA1-411 due to the binding of both the A and B form of PR. Protein A can effectively be used with this product in immunoprecipitation experiments.

MA1-412 detects the A and B forms of progesterone receptor from human, chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

The MA1-412 immunogen is progesterone receptor purified from chick oviduct cytosol.

Reconstitute with PBS.

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pioneer transcription factors, progesterone receptor and Krüppel like transcription factor 4, cooperatively stimulate the bovine herpesvirus 1 ICP0 early promoter and productive late protein expression.

Virus research

Sawant L,Wijesekera N,Jones C

MA1-412 was used in Gel Shift, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to suggest cooperative activation of the bICP0 E promoter by PR and KLF4 correlate with interactions between these pioneer transcription factors.

Thu Oct 15 00:00:00 EDT 2020

Virus research

Sawant L,Wijesekera N,Jones C

Thu Oct 15 00:00:00 EDT 2020

Synergistic Activation of Bovine Herpesvirus 1 Productive Infection and Viral Regulatory Promoters by the Progesterone Receptor and Krüppel-Like Transcription Factor 15.

Journal of virology

El-Mayet FS,El-Habbaa AS,D'Offay J,Jones C

MA1-412 was used in Chromatin immunoprecipitation to suggest that the ability of progesterone and the PR to stimulate productive infection has the potential to promote virus spread in reproductive tissue and induce reproductive failure.

Tue Jan 01 00:00:00 EST 2019

Uterine epithelial morphology and progesterone receptors in a mifepristone-treated viviparous lizard Pseudemoia entrecasteauxii (Squamata: Scincidae) during gestation.

Journal of experimental zoology. Part B, Molecular and developmental evolution

Biazik JM,Parker SL,Murphy CR,Thompson MB

MA1-412 was used in western blot to study the uterine epithelial morphology and progesterone receptors in a viviparous lizard during gestation

Thu Mar 01 00:00:00 EST 2012

Progesterone receptor deficient in chromatin binding has an altered cellular state.

The Journal of biological chemistry

Botos J,Xian W,Smith DF,Smith CL

MA1-412 was used in immunoprecipitation to analyze the effect of progesterone receptors deficient in chromatin binding.

Fri Apr 09 00:00:00 EDT 2004

The Journal of biological chemistry

Felts SJ,Owen BA,Nguyen P,Trepel J,Donner DB,Toft DO

MA1-412 was used in immunoprecipitation to investigate TRAP1 functional properties as a mitochondrial protein

Fri Feb 04 00:00:00 EST 2000

Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.

Molecular and cellular biology

Prapapanich V,Chen S,Smith DF

MA1-412 was used in immunoprecipitation to investigate the important role of Hip's C-terminal region in progesterone receptor assembly

Sun Feb 01 00:00:00 EST 1998

Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids.

The EMBO journal

Hu J,Toft DO,Seeger C

MA1-412 was used in immunoprecipitation to investigate the mechanism of hepadnavirus assembly and viral DNA synthesis

Thu Jan 02 00:00:00 EST 1997

Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein.

Molecular endocrinology (Baltimore, Md.)

Prapapanich V,Chen S,Nair SC,Rimerman RA,Smith DF

MA1-412 was used in immunoprecipitation to characterize the interactions of human p48 in progesterone recepor complexes and with different kinds of heat shock proteins

Mon Apr 01 00:00:00 EST 1996

A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.

The Journal of biological chemistry

Johnson JL,Toft DO

MA1-412 was used in immunoprecipitation to investigate the composition of a new kind of chaperone complex for steroid receptors

Fri Oct 07 00:00:00 EDT 1994

Immunocytochemical study of progesterone receptors in hyperplastic and neoplastic endometrial tissues.

Cancer research

Bergeron C,Ferenczy A,Toft DO,Shyamala G

MA1-412 was used in immunoprecipitation to investigate the expression and distribution of progesterone receptors in hyperplastic and neoplastic human endometrium

Tue Nov 01 00:00:00 EST 1988

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 3 group C member 3; pot. alt. progesterone receptor; putative; PR; Progesterone receptor; unnamed protein product

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Gene Aliases: NR3C3; PGR; PR

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UniProt ID: (Chicken) Q90946, (Human) P06401

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Entrez Gene ID: (Chicken) 396198, (Human) 5241

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding zinc ion binding enzyme binding Hsp70 protein binding chromatin DNA binding sequence-specific DNA binding Hsp90 protein binding steroid hormone binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid binding protein binding ATPase binding

Process(es)

regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter intracellular steroid hormone receptor signaling pathway response to progesterone steroid hormone mediated signaling pathway response to estrogen transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of gene expression positive regulation of transcription from RNA polymerase II promoter

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Progesterone Receptor Monoclonal Antibody (alpha PR-22)

Progesterone Receptor Antibody (MA1-412) in ICC/IF

Product Details

MA1-412

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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