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Prohibitin 2 Monoclonal Antibody (1D9C7)

Prohibitin 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Prohibitin 2 Antibody (66424-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 66424-1-IG) (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin.

Prohibitin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Prohibitin 2 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

66424-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:10,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D9C7

Immunogen

Prohibitin 2 Fusion Protein Ag2977 (1-299 aa encoded by BC014766)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAQNLKDLA GRLPAGPRGM GTALKLLLGA GAVAYGVRES VFTVEGGHRA IFFNRIGGVQ QDTILAEGLH FRIPWFQYPI IYDIRARPRK ISSPTGSKDL QMVNISLRVL SRPNAQELPS MYQRLGLDYE ERVLPSIVNE VLKSVVAKFN ASQLITQRAQ VSLLIRRELT ERAKDFSLIL DDVAITELSF SREYTAAVEA KQVAQQEAQR AQFLVEKAKQ EQRQKIVQAE GEAEAAKMLG EALSKNPGYI KLRKIRAAQN ISKTIATSQN RIYLTADNLV LNLQDESFTR GSDSLIKGKK (1-299 aa encoded by BC014766)

Target Information

PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. This protein functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. It is probably involved in regulating mitochondrial respiration activity and in aging.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rutin Attenuates Oxidative Stress Via PHB2-Mediated Mitophagy in MPP⁺-Induced SH-SY5Y Cells.

Neurotoxicity research

Lai X,Zhang Y,Wu J,Shen M,Yin S,Yan J

Thu Jun 01 00:00:00 EDT 2023

Rutin Attenuates Oxidative Stress Via PHB2-Mediated Mitophagy in MPP⁺-Induced SH-SY5Y Cells.

Neurotoxicity research

Lai X,Zhang Y,Wu J,Shen M,Yin S,Yan J

Thu Jun 01 00:00:00 EDT 2023

REC drives recombination to repair double-strand breaks in animal mtDNA.

The Journal of cell biology

Klucnika A,Mu P,Jezek J,McCormack M,Di Y,Bradshaw CR,Ma H

Mon Jan 02 00:00:00 EST 2023

Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.

Biosensors & bioelectronics

Yamagishi A,Mizusawa M,Uchida K,Iijima M,Kuroda S,Fukazawa K,Ishihara K,Nakamura C

Tue Nov 15 00:00:00 EST 2022

Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.

Biosensors & bioelectronics

Yamagishi A,Mizusawa M,Uchida K,Iijima M,Kuroda S,Fukazawa K,Ishihara K,Nakamura C

Tue Nov 15 00:00:00 EST 2022

Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.

Biosensors & bioelectronics

Yamagishi A,Mizusawa M,Uchida K,Iijima M,Kuroda S,Fukazawa K,Ishihara K,Nakamura C

Tue Nov 15 00:00:00 EST 2022

Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.

Biosensors & bioelectronics

Yamagishi A,Mizusawa M,Uchida K,Iijima M,Kuroda S,Fukazawa K,Ishihara K,Nakamura C

Tue Nov 15 00:00:00 EST 2022

Nonreceptor Tyrosine Kinase c-Abl-Mediated PHB2 Phosphorylation Aggravates Mitophagy Disorder in Parkinson's Disease Model.

Oxidative medicine and cellular longevity

Zhang Y,Wu J,Jin W,Shen M,Yin S,Lai X,Ma H,Jiang M,Sun D,Yan J

Tue Nov 22 00:00:00 EST 2022

Discovery of bactericides as an acute mitochondrial membrane damage inducer.

Molecular biology of the cell

Houston R,Sekine Y,Larsen MB,Murakami K,Mullett SJ,Wendell SG,Narendra DP,Chen BB,Sekine S

Mon Nov 01 00:00:00 EDT 2021

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M,Friedman J,Jost M,Xu J,Yamada J,Kampmann M,Horlbeck MA,Salemi MR,Phinney BS,Weissman JS,Nunnari J

Wed May 26 00:00:00 EDT 2021

A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids.

PloS one

Nasonovs A,Garcia-Diaz M,Bogenhagen DF

Tue Oct 19 00:00:00 EDT 2021

Fluoride induced mitochondrial impairment and PINK1-mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies.

Environmental pollution (Barking, Essex : 1987)

Liang C,Gao Y,He Y,Han Y,Manthari RK,Tikka C,Chen C,Wang J,Zhang J

Wed Jan 01 00:00:00 EST 2020

Fluoride induced mitochondrial impairment and PINK1-mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies.

Environmental pollution (Barking, Essex : 1987)

Liang C,Gao Y,He Y,Han Y,Manthari RK,Tikka C,Chen C,Wang J,Zhang J

Wed Jan 01 00:00:00 EST 2020

Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.

Viruses

Su W,Huang S,Zhu H,Zhang B,Wu X

Wed Apr 08 00:00:00 EDT 2020

Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.

Viruses

Su W,Huang S,Zhu H,Zhang B,Wu X

Wed Apr 08 00:00:00 EDT 2020

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Life science alliance

Richter U,Ng KY,Suomi F,Marttinen P,Turunen T,Jackson C,Suomalainen A,Vihinen H,Jokitalo E,Nyman TA,Isokallio MA,Stewart JB,Mancini C,Brusco A,Seneca S,Lombès A,Taylor RW,Battersby BJ

Fri Feb 01 00:00:00 EST 2019

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

EMBO reports

Furuya N,Kakuta S,Sumiyoshi K,Ando M,Nonaka R,Suzuki A,Kazuno S,Saiki S,Hattori N

Sat Dec 01 00:00:00 EST 2018

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

EMBO reports

Furuya N,Kakuta S,Sumiyoshi K,Ando M,Nonaka R,Suzuki A,Kazuno S,Saiki S,Hattori N

Sat Dec 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: B-cell associated protein; B-cell receptor associated protein 37; B-cell receptor-associated protein 37; B-cell receptor-associated protein BAP37; BAP-37; D-prohibitin; MGC117268; PHB2; phb2 {ECO:0000250|UniProtKB:Q99623}; Prohibitin-2; Repressor of estrogen receptor activity

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Gene Aliases: AU044498; BAP; Bap-37; Bap37; Bcap27; BCAP37; hBAP; p22; PHB2; PNAS-141; REA

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UniProt ID: (Human) Q99623, (Mouse) O35129, (Rat) Q5XIH7

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Entrez Gene ID: (Human) 11331, (Mouse) 12034, (Rat) 114766

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Function(s)

protein binding protein C-terminus binding estrogen receptor binding amide binding protein N-terminus binding chromatin binding molecular_function

Process(es)

protein import into nucleus, translocation transcription, DNA-templated regulation of transcription, DNA-templated mitochondrion organization sister chromatid cohesion positive regulation of exit from mitosis negative regulation of intracellular estrogen receptor signaling pathway negative regulation of mammary gland epithelial cell proliferation negative regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of transcription, DNA-templated protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity mammary gland branching involved in thelarche mammary gland alveolus development regulation of branching involved in mammary gland duct morphogenesis positive regulation of ERK1 and ERK2 cascade positive regulation of cell cycle G1/S phase transition regulation of complement activation biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Prohibitin 2 Monoclonal Antibody (1D9C7)

Prohibitin 2 Antibody (66424-1-IG) in ICC/IF

Product Details

66424-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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