Proteintech

Prohibitin Polyclonal Antibody

Prohibitin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

Prohibitin Antibody (10787-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Prohibitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Prohibitin Antibody in Western Blot (WB)

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Product Details

10787-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Prohibitin Fusion Protein Ag1240 (1-272 aa encoded by BC013401)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAAKVFESI GKFGLALAVA GGVVNSALYN VDAGHRAVIF DRFRGVQDIV VGEGTHFLIP WVQKPIIFDC RSRPRNVPVI TGSKDLQNVN ITLRILFRPV ASQLPRIFTS IGEDYDERVL PSITTEILKS VVARFDAGEL ITQRELVSRQ VSDDLTERAA TFGLILDDVS LTHLTFGKEF TEAVEAKQVA QQEAERARFV VEKAEQQKKA AIISAEGDSK AAELIANSLA TAGDGLIELR KLEAAEDIAY QLSRSRNITY LPAGQSVLLQ LPQ (1-272 aa encoded by BC013401)

Target Information

Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury.

Hepatology (Baltimore, Md.)

Chen L,Dong J,Liao S,Wang S,Wu Z,Zuo M,Liu B,Yan C,Chen Y,He H,Meng Q,Song Z

Tue Nov 01 00:00:00 EDT 2022

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

International journal of molecular sciences

Naki M,Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

Fri May 06 00:00:00 EDT 2022

DJ-1 Deficiency in Hepatocytes Improves Liver Ischemia-Reperfusion Injury by Enhancing Mitophagy.

Cellular and molecular gastroenterology and hepatology

Xu M,Hang H,Huang M,Li J,Xu D,Jiao J,Wang F,Wu H,Sun X,Gu J,Kong X,Gao Y

Thu Jan 27 00:00:00 EST 2022

Long Non-Coding RNA CD27-AS1-208 Facilitates Melanoma Progression by Activating STAT3 Pathway.

Frontiers in oncology

Ma J,Shi Q,Guo S,Xu P,Yi X,Yang Y,Zhang W,Liu Y,Liu L,Yue Q,Zhao T,Gao T,Guo W,Li C

Tue Feb 01 00:00:00 EST 2022

Involvement of miR-27a-3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress.

Journal of cellular physiology

Wu L,Wang Q,Guo F,Ma X,Wang J,Zhao Y,Yan Y,Qin G

Mon Feb 01 00:00:00 EST 2021

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

eLife

Dennerlein S,Poerschke S,Oeljeklaus S,Wang C,Richter-Dennerlein R,Sattmann J,Bauermeister D,Hanitsch E,Stoldt S,Langer T,Jakobs S,Warscheid B,Rehling P

Fri Dec 31 00:00:00 EST 2021

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Autophagy

Yan C,Gong L,Chen L,Xu M,Abou-Hamdan H,Tang M,Désaubry L,Song Z

Sun Mar 01 00:00:00 EST 2020

Regulatory effects of Prohibitin 1 on proliferation and apoptosis of pulmonary arterial smooth muscle cells in monocrotaline-induced PAH rats.

Life sciences

Cao YY,Ba HX,Li Y,Tang SY,Luo ZQ,Li XH

Mon Jun 01 00:00:00 EDT 2020

Regulatory effects of Prohibitin 1 on proliferation and apoptosis of pulmonary arterial smooth muscle cells in monocrotaline-induced PAH rats.

Life sciences

Cao YY,Ba HX,Li Y,Tang SY,Luo ZQ,Li XH

Mon Jun 01 00:00:00 EDT 2020

IFN-γ restores the impaired function of RNase L and induces mitochondria-mediated apoptosis in lung cancer.

Cell death & disease

Yin H,Jiang Z,Wang S,Zhang P

Mon Sep 09 00:00:00 EDT 2019

Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.

Cellular microbiology

Yahiro K,Ogura K,Terasaki Y,Satoh M,Miyagi S,Terasaki M,Yamasaki E,Moss J

Thu Aug 01 00:00:00 EDT 2019

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

EBioMedicine

Han Y,Wu P,Wang Z,Zhang Z,Sun S,Liu J,Gong S,Gao P,Iwakuma T,Molina-Vila MA,Chen BP,Zhang Y,Ji T,Mo Q,Chen P,Hu J,Wang S,Zhou J,Lu H,Gao Q

Fri Feb 01 00:00:00 EST 2019

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

EBioMedicine

Han Y,Wu P,Wang Z,Zhang Z,Sun S,Liu J,Gong S,Gao P,Iwakuma T,Molina-Vila MA,Chen BP,Zhang Y,Ji T,Mo Q,Chen P,Hu J,Wang S,Zhou J,Lu H,Gao Q

Fri Feb 01 00:00:00 EST 2019

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

EBioMedicine

Han Y,Wu P,Wang Z,Zhang Z,Sun S,Liu J,Gong S,Gao P,Iwakuma T,Molina-Vila MA,Chen BP,Zhang Y,Ji T,Mo Q,Chen P,Hu J,Wang S,Zhou J,Lu H,Gao Q

Fri Feb 01 00:00:00 EST 2019

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

EBioMedicine

Han Y,Wu P,Wang Z,Zhang Z,Sun S,Liu J,Gong S,Gao P,Iwakuma T,Molina-Vila MA,Chen BP,Zhang Y,Ji T,Mo Q,Chen P,Hu J,Wang S,Zhou J,Lu H,Gao Q

Fri Feb 01 00:00:00 EST 2019

NAD⁺ repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-β in endothelial cells through improving mitochondrial unfolded protein response.

The international journal of biochemistry & cell biology

Zhang M,Weng H,Zheng J

Sun Dec 01 00:00:00 EST 2019

Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P₂ and extended synaptotagmins.

Science China. Life sciences

Xiao J,Luo J,Hu A,Xiao T,Li M,Kong Z,Jiang L,Zhou Z,Liao Y,Xie C,Chu B,Miao H,Li B,Shi X,Song BL

Sun Sep 01 00:00:00 EDT 2019

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Cell death and differentiation

Li H,Ruan Y,Zhang K,Jian F,Hu C,Miao L,Gong L,Sun L,Zhang X,Chen S,Chen H,Liu D,Song Z

Tue Mar 01 00:00:00 EST 2016

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Cell death and differentiation

Li H,Ruan Y,Zhang K,Jian F,Hu C,Miao L,Gong L,Sun L,Zhang X,Chen S,Chen H,Liu D,Song Z

Tue Mar 01 00:00:00 EST 2016

Cholesterol transport through lysosome-peroxisome membrane contacts.

Cell

Chu BB,Liao YC,Qi W,Xie C,Du X,Wang J,Yang H,Miao HH,Li BL,Song BL

Thu Apr 09 00:00:00 EDT 2015

Cholesterol transport through lysosome-peroxisome membrane contacts.

Cell

Chu BB,Liao YC,Qi W,Xie C,Du X,Wang J,Yang H,Miao HH,Li BL,Song BL

Thu Apr 09 00:00:00 EDT 2015

Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.

Evidence-based complementary and alternative medicine : eCAM

Guo CC,Zheng LH,Fu JY,Zhu JH,Zhou YX,Zeng T,Zhou ZK

Wed Nov 11 00:00:00 EST 2015

Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.

Evidence-based complementary and alternative medicine : eCAM

Guo CC,Zheng LH,Fu JY,Zhu JH,Zhou YX,Zeng T,Zhou ZK

Wed Nov 11 00:00:00 EST 2015

A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas.

Diagnostic pathology

Yang MS,Wang HS,Wang BS,Li WH,Pang ZF,Zou BK,Zhang X,Shi XT,Mu DB,Zhang DX,Gao YS,Sun XW,Xia SJ

Mon Apr 15 00:00:00 EDT 2013

A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas.

Diagnostic pathology

Yang MS,Wang HS,Wang BS,Li WH,Pang ZF,Zou BK,Zhang X,Shi XT,Mu DB,Zhang DX,Gao YS,Sun XW,Xia SJ

Mon Apr 15 00:00:00 EDT 2013

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Journal of proteome research

Liu C,Zhang A,Guo J,Yang J,Zhou H,Chen H,Jin M

Fri Nov 02 00:00:00 EDT 2012

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Journal of proteome research

Liu C,Zhang A,Guo J,Yang J,Zhou H,Chen H,Jin M

Fri Nov 02 00:00:00 EDT 2012

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Human molecular genetics

McCray BA,Skordalakes E,Taylor JP

Mon Mar 15 00:00:00 EDT 2010

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Human molecular genetics

McCray BA,Skordalakes E,Taylor JP

Mon Mar 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1

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Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1

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UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779

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Entrez Gene ID: (Human) 5245, (Mouse) 18673, (Rat) 25344

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding complement component C3a binding complement component C3b binding protein binding protein C-terminus binding enzyme binding proteinase activated receptor binding histone deacetylase binding transcription regulatory region DNA binding molecular_function

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Prohibitin Polyclonal Antibody

Prohibitin Antibody (10787-1-AP) in ICC/IF

Product Details

10787-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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