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Invitrogen
Prohibitin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Prohibitin Antibody (PA5-17325) in ICC/IF
Immunofluorescence analysis of Prohibitin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Prohibitin Polyclonal Antibody (Product # PA5-17325) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More
Product Details
PA5-17325
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Ser252 of human PHB1
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10977876
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1
Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1
UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779
Entrez Gene ID: (Human) 5245, (Mouse) 18673, (Rat) 25344
RNA polymerase II transcription factor activity, sequence-specific DNA binding
complement component C3a binding
complement component C3b binding
protein binding
protein C-terminus binding
enzyme binding
proteinase activated receptor binding
histone deacetylase binding
transcription regulatory region DNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
regulation of transcription, DNA-templated
mitochondrion organization
signal transduction
negative regulation of cell proliferation
negative regulation of transcription by competitive promoter binding
histone deacetylation
negative regulation of cell growth
negative regulation of protein catabolic process
regulation of apoptotic process
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of complement activation
protein stabilization
progesterone receptor signaling pathway
negative regulation of androgen receptor signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to interleukin-6
DNA biosynthetic process
negative regulation of glucocorticoid receptor signaling pathway
transcription from RNA polymerase II promoter
negative regulation of apoptotic process
ovarian follicle development
ovarian follicle atresia
organ regeneration
response to cytokine
response to immobilization stress
response to drug
response to peptide hormone
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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