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Prohibitin Antibody (PA5-85375) in ICC/IF
Prohibitin Polyclonal Antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: Prohibitin protein stained by Prohibitin Polyclonal Antibody (Product # PA5-85375) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-85375
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Prohibitin.
Conjugate
Form
Liquid
Concentration
0.91 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792517
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (94%), Xenopus laevis (89%), Dog (100%), Cat (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Positive Control: IMR32, U87-MG, Neuro2A, GL261, C8D30, PC-12, Rat2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1
Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1
UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779
Entrez Gene ID: (Human) 5245, (Mouse) 18673, (Rat) 25344
RNA polymerase II transcription factor activity, sequence-specific DNA binding
complement component C3a binding
complement component C3b binding
protein binding
protein C-terminus binding
enzyme binding
proteinase activated receptor binding
histone deacetylase binding
transcription regulatory region DNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
regulation of transcription, DNA-templated
mitochondrion organization
signal transduction
negative regulation of cell proliferation
negative regulation of transcription by competitive promoter binding
histone deacetylation
negative regulation of cell growth
negative regulation of protein catabolic process
regulation of apoptotic process
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of complement activation
protein stabilization
progesterone receptor signaling pathway
negative regulation of androgen receptor signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to interleukin-6
DNA biosynthetic process
negative regulation of glucocorticoid receptor signaling pathway
transcription from RNA polymerase II promoter
negative regulation of apoptotic process
ovarian follicle development
ovarian follicle atresia
organ regeneration
response to cytokine
response to immobilization stress
response to drug
response to peptide hormone
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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