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Invitrogen
Protein APC Recombinant Superclonal Antibody (1HCLC)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Protein APC Antibody (710859) in ICC/IF
Immunofluorescence analysis of Protein APC was performed using 70% confluent log phase HT29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Protein APC Recombinant Polyclonal Antibody (1HCLC) (Product # 710859) at 1:100 in 0.1% BSA, incubated at 4... View More
Product Details
710859
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
1HCLC
Immunogen
Protein corresponding to Human APC (aa 2-310)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633099
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Pig and Bovine.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenomatosis polyposis coli tumor suppressor; Adenomatous polyposis coli protein; Deleted in polyposis 2.5; FPC; Protein APC; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli; WNT signaling pathway regulator
Gene Aliases: APC; BTPS2; DP2; DP2.5; DP3; GS; PPP1R46
UniProt ID: (Human) P25054
Entrez Gene ID: (Human) 324
mitotic cytokinesis
cell fate specification
protein complex assembly
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
negative regulation of microtubule depolymerization
cell cycle arrest
mitotic spindle assembly checkpoint
cell adhesion
establishment or maintenance of cell polarity
pattern specification process
negative regulation of cell proliferation
Wnt signaling pathway
cell migration
positive regulation of cell migration
pancreas development
neuron projection development
positive regulation of pseudopodium assembly
regulation of microtubule-based process
response to drug
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of cell differentiation
positive regulation of protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of attachment of spindle microtubules to kinetochore
canonical Wnt signaling pathway
bicellular tight junction assembly
negative regulation of canonical Wnt signaling pathway
response to alcohol
beta-catenin destruction complex assembly
beta-catenin destruction complex disassembly
cellular response to nerve growth factor stimulus
regulation of glutamate metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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