Invitrogen

CD3e Monoclonal Antibody (UCHT1), Qdot™ 605

FIGURE: 1 / 1

CD3e Antibody (Q10054) in Flow

Qdot® 605 anti-human CD3 conjugate 605/20 band pass filter

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Product Details

Q10054

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 11 publications 11 publications

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Immunogen

Human CD3e

Conjugate

Qdot™ 605 Qdot™ 605 Qdot™ 605

Excitation/Emission Max

300/603 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11180459

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Duration of Humoral and Cellular Immunity 8 Years After Administration of Reduced Doses of the 17DD-Yellow Fever Vaccine.

Frontiers in immunology

da Costa-Rocha IA,Campi-Azevedo AC,Peruhype-Magalhães V,Coelho-Dos-Reis JG,Fradico JRB,Souza-Lopes T,Reis LR,Freire LC,Costa-Pereira C,Mambrini JVM,Maia MLS,de Lima SMB,de Noronha TG,Xavier JR,Camacho LAB,de Albuquerque EM,Farias RHG,de Castro TDM,Homma A,Romano APM,Domingues CM,Martins RM,Teixeira-Carvalho A,Martins-Filho OA

Q10054 was used in In vitro Assay to support the regular use of dose sparing strategy for YF vaccine in adults.

Mon Oct 19 00:00:00 EDT 2020

Multi-parameter approach to evaluate the timing of memory status after 17DD-YF primary vaccination.

PLoS neglected tropical diseases

Costa-Pereira C,Campi-Azevedo AC,Coelho-Dos-Reis JG,Peruhype-Magalhães V,Araújo MSS,do Vale Antonelli LR,Fonseca CT,Lemos JA,Malaquias LCC,de Souza Gomes M,Rodrigues Amaral L,Rios M,Chancey C,Persi HR,Pereira JM,de Sousa Maia ML,Freire MDS,Martins RM,Homma A,Simões M,Yamamura AY,Farias RHG,Romano APM,Domingues CM,Tauil PL,Vasconcelos PFC,Caldas IR,Camacho LA,Teixeira-Carvalho A,Martins-Filho OA

Q10054 was used in Flow cytometry/Cell sorting to identify a minimum set of phenotypic/functional memory-related biomarkers for post-vaccination follow-up upon yellow fever vaccination.

Fri Jun 01 00:00:00 EDT 2018

IL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion.

JCI insight

Le Saout C,Luckey MA,Villarino AV,Smith M,Hasley RB,Myers TG,Imamichi H,Park JH,O'Shea JJ,Lane HC,Catalfamo M

Q10054 was used in Flow cytometry/Cell sorting to propose a model in which T cells undergoing lymphopenia-induced proliferation upregulate STAT1 protein, switching on an alternate IL-7-dependent program.

Thu Nov 16 00:00:00 EST 2017

CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.

PLoS pathogens

Manivannan K,Rowan AG,Tanaka Y,Taylor GP,Bangham CR

Q10054 was used in Flow cytometry/Cell sorting to show that cell adhesion molecule 1 expression is associated with enhanced susceptibility of infected cells to cytotoxic T lymphocyte lysis.

Fri Apr 01 00:00:00 EDT 2016

Booster dose after 10 years is recommended following 17DD-YF primary vaccination.

Human vaccines & immunotherapeutics

Campi-Azevedo AC,Costa-Pereira C,Antonelli LR,Fonseca CT,Teixeira-Carvalho A,Villela-Rezende G,Santos RA,Batista MA,Campos FM,Pacheco-Porto L,Melo Júnior OA,Hossell DM,Coelho-dos-Reis JG,Peruhype-Magalhães V,Costa-Silva MF,de Oliveira JG,Farias RH,Noronha TG,Lemos JA,von Doellinger Vdos R,Simões M,de Souza MM,Malaquias LC,Persi HR,Pereira JM,Martins JA,Dornelas-Ribeiro M,Vinhas Ade A,Alves TR,Maia Mde L,Freire Mda S,Martins Rde M,Homma A,Romano AP,Domingues CM,Tauil PL,Vasconcelos PF,Rios M,Caldas IR,Camacho LA,Martins-Filho OA

Q10054 was used in flow cytometry to assess the efficacy of a single vaccination of the Yellow Fever vaccine

Tue Dec 13 00:00:00 EST 2016

Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?

PloS one

Stikvoort A,Sundin M,Uzunel M,Gertow J,Sundberg B,Schaffer M,Mattsson J,Uhlin M

Q10054 was used in flow cytometry to test if the two hematopoietic systems in patients with mixed chimerism remain functional and study immunological differences

Mon Jul 17 00:00:00 EDT 2017

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

Q10054 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets

Thu Dec 18 00:00:00 EST 2014

Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.

European journal of immunology

Dintwe OB,Day CL,Smit E,Nemes E,Gray C,Tameris M,McShane H,Mahomed H,Hanekom WA,Scriba TJ

Q10054 was used in flow cytometry to characterize T cells after MVA85A vaccination

Sun Sep 01 00:00:00 EDT 2013

CD4+ T-cell responses among adults and young children in response to Streptococcus pneumoniae and Haemophilus influenzae vaccine candidate protein antigens.

Vaccine

Sharma SK,Roumanes D,Almudevar A,Mosmann TR,Pichichero ME

Q10054 was used in flow cytometry to determine cytokine profiles of CD4(+) T-helper (h) cells in adults and young children vaccinated with the antigens PspA, PcpA, PhtD, PhtE, Ply, LytB of Streptococcus pneumonia and protein D and OMP26 of non-typeable Haemophilus influenza.

Wed Jun 26 00:00:00 EDT 2013

Expansion of HIV-specific T follicular helper cells in chronic HIV infection.

The Journal of clinical investigation

Lindqvist M,van Lunzen J,Soghoian DZ,Kuhl BD,Ranasinghe S,Kranias G,Flanders MD,Cutler S,Yudanin N,Muller MI,Davis I,Farber D,Hartjen P,Haag F,Alter G,Schulze zur Wiesch J,Streeck H

Q10054 was used in Flow cytometry/Cell sorting to assess HIV-specific T follicular helper cell responses in the lymph nodes of treatment-naive and antiretroviral-treated HIV-infected individuals.

Sat Sep 01 00:00:00 EDT 2012

Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

Vaccine

Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR

Q10054 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.

Fri Jun 29 00:00:00 EDT 2012

Uncoupling of proliferation and cytokines from suppression within the CD4+CD25+Foxp3+ T-cell compartment in the 1st year of human type 1 diabetes.

Diabetes

Hughson A,Bromberg I,Johnson B,Quataert S,Jospe N,Fowell DJ

Q10054 was used in Flow cytometry/Cell sorting to determine the relative proportions of effector and regulatory T cells in the CD4+CD25+Foxp3+ T-cell compartment in varying type 1 diabetes disease states.

Mon Aug 01 00:00:00 EDT 2011

Association of human TLR1 and TLR6 deficiency with altered immune responses to BCG vaccination in South African infants.

PLoS pathogens

Randhawa AK,Shey MS,Keyser A,Peixoto B,Wells RD,de Kock M,Lerumo L,Hughes J,Hussey G,Hawkridge A,Kaplan G,Hanekom WA,Hawn TR

Q10054 was used in Flow cytometry/Cell sorting to provide evidence that functionally defined innate immune gene variants are associated with the development of adaptive immune responses after in vivo vaccination against a bacterial pathogen in humans.

Mon Aug 01 00:00:00 EDT 2011

CD4 and CD8 T cell immune activation during chronic HIV infection: roles of homeostasis, HIV, type I IFN, and IL-7.

Journal of immunology (Baltimore, Md. : 1950)

Catalfamo M,Wilhelm C,Tcheung L,Proschan M,Friesen T,Park JH,Adelsberger J,Baseler M,Maldarelli F,Davey R,Roby G,Rehm C,Lane C

Q10054 was used in flow cytometry and western blot to investigate the regulation of the CD4 and CD8 T cell pools during HIV infection

Tue Feb 15 00:00:00 EST 2011

CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.

Vaccine

Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ

Q10054 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.

Thu Mar 03 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD3e Monoclonal Antibody (UCHT1), Qdot™ 605

CD3e Antibody (Q10054) in Flow

Product Details

Q10054

Flow Cytometry (Flow)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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