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Invitrogen
RAB11A Recombinant Rabbit Monoclonal Antibody (3H18L5), Alexa Fluor™ 488
FIGURE: 1 / 1
RAB11A Antibody (MA7-00184-A488) in ICC/IF
Immunofluorescent analysis of Rab11 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Rab11 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA7-00184-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Im... View More
Product Details
MA7-00184-A488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3H18L5
Immunogen
A peptide corresponding to amino acids 179-194 of human Rab11.
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2663041
Product Specific Information
This antibody is predicted to react with bovine, chicken, equine, mouse, primate, rat, Xenopus and zebrafish based on sequence homology
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Rab11A is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11A mRNA has been detected in a variety of tissues including brain, testis, spleen, heart, and gastrointestinal mucosa. In the gastric fundus the Rab11A protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11A protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations. Recent studies have shown Rab11A is essential for resecretion of alpha-synuclein out of neuronal cells via endosome recycling and is required for exocytosis of discoidal/fusiform vesicles of bladder umbrella cells. Rab11A is also implicated in biogenesis of Birbeck granules and endosomal activation of the PI3K/AKT signaling pathway via G beta gamma trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: RAB 11A, member oncogene family; Rab-11; Ras-related protein Rab-11A; YL8
Gene Aliases: RAB11; RAB11A; YL8
UniProt ID: (Human) P62491
Entrez Gene ID: (Human) 8766
cytokinesis
renal water homeostasis
mitotic metaphase plate congression
small GTPase mediated signal transduction
positive regulation of epithelial cell migration
regulation of multivesicular body size
positive regulation of G2/M transition of mitotic cell cycle
protein transport
vesicle-mediated transport
astral microtubule organization
neuron projection development
melanosome transport
multivesicular body assembly
positive regulation of axon extension
regulation of long-term neuronal synaptic plasticity
plasma membrane to endosome transport
regulation of protein transport
establishment of vesicle localization
regulation of vesicle-mediated transport
establishment of protein localization to organelle
protein localization to plasma membrane
establishment of protein localization to membrane
mitotic spindle assembly
exosomal secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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