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RAB27A Antibody (PA5-44580) in ICC/IF
Immunofluorescence analysis of RAB27A was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAB27A Polyclonal Antibody (Product # PA5-44580) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1... View More
Product Details
PA5-44580
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the middle region of human RAB27A
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2608618
Product Specific Information
Peptide sequence: SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHASTDQL SEEKEKGACG
Sequence homology: Cow: 79%; Goat: 79%; Horse: 79%; Human: 100%; Mouse: 86%; Rat: 93%; Sheep: 79%
Target Information
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein Ram; mutant Ras-related protein Rab-27A; Rab-27; Ras-related protein Rab-27A
Gene Aliases: GS2; HsT18676; RAB27; RAB27A; RAM
UniProt ID: (Human) P51159
Entrez Gene ID: (Human) 5873
protein targeting
exocytosis
small GTPase mediated signal transduction
blood coagulation
positive regulation of gene expression
antigen processing and presentation
melanocyte differentiation
melanosome localization
melanosome transport
multivesicular body organization
cytotoxic T cell degranulation
natural killer cell degranulation
positive regulation of exocytosis
synaptic vesicle transport
positive regulation of phagocytosis
multivesicular body sorting pathway
complement-dependent cytotoxicity
positive regulation of regulated secretory pathway
positive regulation of reactive oxygen species biosynthetic process
positive regulation of constitutive secretory pathway
exosomal secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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