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RAB32 Polyclonal Antibody

View all (10) RAB32 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (11)

RAB32 Antibody in Immunocytochemistry (ICC/IF)

RAB32 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

RAB32 Antibody (10999-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (Product # 10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAB32 Antibody in Immunocytochemistry (ICC/IF)

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB32 Antibody in Western Blot (WB)

RAB32 Antibody in Western Blot (WB)

RAB32 Antibody in Western Blot (WB)

Product Image Gallery

RAB32 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (Product # 10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using (Product # 10999-1-AP) RAB32 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using (Product # 10999-1-AP) RAB32 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10999-1-AP (RAB32 antibody) at dilution of 1:50 (under 10x lens).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 10999-1-AP (RAB32 antibody) at dilution of 1:50 (under 40x lens).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 10999-1-AP (RAB32 antibody) at dilution of 1:50 (under 10x lens).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 10999-1-AP (RAB32 antibody) at dilution of 1:50 (under 40x lens).

RAB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 10999-1-AP (RAB32 antibody) at dilution of 1:50 (under 10x lens).

RAB32 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10999-1-AP) RAB32 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

RAB32 Antibody in Western Blot (WB) — Human colon tissue were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RAB32 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

10999-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAB32 Fusion Protein Ag1450 (1-225 aa encoded by BC015061)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAGGGAGDP GLGAAAAPAP ETREHLFKVL VIGELGVGKT SIIKRYVHQL FSQHYRATIG VDFALKVLNW DSRTLVRLQL WDIAGQERFG NMTRVYYKEA VGAFVVFDIS RSSTFEAVLK WKSDLDSKVH LPNGSPIPAV LLANKCDQNK DSSQSPSQVD QFCKEHGFAG WFETSAKDNI NIEEAARFLV EKILVNHQSF PNEENDVDKI KLDQETLRAE NKSQCC (1-225 aa encoded by B C015061)

Target Information

RAB32 acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission.

Cell death & disease

Chen P,Lu Y,He B,Xie T,Yan C,Liu T,Wu S,Yeh Y,Li Z,Huang W,Zhang X

Wed Mar 15 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: Ras-related protein Rab-32

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Gene Aliases: RAB32

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UniProt ID: (Human) Q13637

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Entrez Gene ID: (Human) 10981

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GTPase activity protein binding GTP binding GTP-dependent protein binding AP-2 adaptor complex binding AP-1 adaptor complex binding AP-3 adaptor complex binding BLOC-2 complex binding

Process(es)

small GTPase mediated signal transduction antigen processing and presentation endosome to melanosome transport phagosome maturation melanosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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