Proteintech

RAB35 Polyclonal Antibody

RAB35 Antibody in Immunocytochemistry (ICC/IF)

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RAB35 Antibody (11329-2-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 11329-2-AP (RAB35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB35 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11329-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 32 publications 32 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 10 publications 10 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAB35 Fusion Protein Ag1872 (1-201 aa encoded by BC015931)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MARDYDHLF KLLIIGDSGV GKSSLLLRFA DNTFSGSYIT TIGVDFKIRT VEINGEKVKL QIWDTAGQER FRTITSTYYR GTHGVIVVYD VTSAESFVNV KRWLHEINQN CDDVCRILVG NKNDDPERKV VETEDAYKFA GQMGIQLFET SAKENVNVEE MFNCITELVL RAKKDNLAKQ QQQQQNDVVK LTKNSKRKKR CC (1-201 aa encoded by BC015931)

Target Information

RAB35 possesses GTPase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DDX3 suppresses hepatocellular carcinoma progression through modulating the secretion and composition of exosome.

American journal of cancer research

Su YY,Chao CH,Hsu HY,Li HK,Wang YL,Wu Lee YH,Mai RT

Mon Jun 12 00:00:00 EDT 2023

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.

Cell reports

Matsui T,Sakamaki Y,Nakashima S,Fukuda M

Tue May 31 00:00:00 EDT 2022

TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.

International journal of molecular sciences

Zhang T,Zhao L,Han L,Li Y,Hu L,Wang H,Zou F

Sun Jun 12 00:00:00 EDT 2022

Chronic pain causes Tau-mediated hippocampal pathology and memory deficits.

Molecular psychiatry

Guerreiro SR,Guimarães MR,Silva JM,Dioli C,Vamvaka-Iakovou A,Sousa R,Gomes P,Megalokonomou A,Campos-Marques C,Cunha AM,Almeida A,Sousa N,Leite-Almeida H,Sotiropoulos I

Tue Nov 01 00:00:00 EDT 2022

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Journal of cell science

Iannantuono NVG,Emery G

Tue Jun 15 00:00:00 EDT 2021

Improved Small Extracellular Vesicle Secretion of Rat Adipose-Derived Stem Cells by Microgrooved Substrates through Upregulation of the ESCRT-III-Associated Protein Alix.

Advanced healthcare materials

Ji Y,Han W,Fu X,Li J,Wu Q,Wang Y

Sun Aug 01 00:00:00 EDT 2021

Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.

Cell death & disease

Zhuravleva V,Vaz-Silva J,Zhu M,Gomes P,Silva JM,Sousa N,Sotiropoulos I,Waites CL

Wed Dec 08 00:00:00 EST 2021

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Cell metabolism

Mukherjee C,Kling T,Russo B,Miebach K,Kess E,Schifferer M,Pedro LD,Weikert U,Fard MK,Kannaiyan N,Rossner M,Aicher ML,Goebbels S,Nave KA,Krämer-Albers EM,Schneider A,Simons M

Tue Aug 04 00:00:00 EDT 2020

Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.

The Journal of biological chemistry

Kinoshita R,Homma Y,Fukuda M

Fri Mar 13 00:00:00 EDT 2020

Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.

The Journal of biological chemistry

Kinoshita R,Homma Y,Fukuda M

Fri Mar 13 00:00:00 EDT 2020

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Science advances

Bonet-Ponce L,Beilina A,Williamson CD,Lindberg E,Kluss JH,Saez-Atienzar S,Landeck N,Kumaran R,Mamais A,Bleck CKE,Li Y,Cookson MR

Sun Nov 01 00:00:00 EDT 2020

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Science advances

Bonet-Ponce L,Beilina A,Williamson CD,Lindberg E,Kluss JH,Saez-Atienzar S,Landeck N,Kumaran R,Mamais A,Bleck CKE,Li Y,Cookson MR

Sun Nov 01 00:00:00 EDT 2020

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

Nature cell biology

Wallroth A,Koch PA,Marat AL,Krause E,Haucke V

Sun Sep 01 00:00:00 EDT 2019

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Nature cell biology

Clancy JW,Zhang Y,Sheehan C,D'Souza-Schorey C

Mon Jul 01 00:00:00 EDT 2019

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.

Nature communications

Koike S,Jahn R

Mon Apr 08 00:00:00 EDT 2019

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

EMBO reports

Furuya N,Kakuta S,Sumiyoshi K,Ando M,Nonaka R,Suzuki A,Kazuno S,Saiki S,Hattori N

Sat Dec 01 00:00:00 EST 2018

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

EMBO reports

Furuya N,Kakuta S,Sumiyoshi K,Ando M,Nonaka R,Suzuki A,Kazuno S,Saiki S,Hattori N

Sat Dec 01 00:00:00 EST 2018

LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation.

Nature communications

Bae EJ,Kim DK,Kim C,Mante M,Adame A,Rockenstein E,Ulusoy A,Klinkenberg M,Jeong GR,Bae JR,Lee C,Lee HJ,Lee BD,Di Monte DA,Masliah E,Lee SJ

Mon Aug 27 00:00:00 EDT 2018

Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration.

Molecular neurodegeneration

Jeong GR,Jang EH,Bae JR,Jun S,Kang HC,Park CH,Shin JH,Yamamoto Y,Tanaka-Yamamoto K,Dawson VL,Dawson TM,Hur EM,Lee BD

Tue Feb 13 00:00:00 EST 2018

Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration.

Molecular neurodegeneration

Jeong GR,Jang EH,Bae JR,Jun S,Kang HC,Park CH,Shin JH,Yamamoto Y,Tanaka-Yamamoto K,Dawson VL,Dawson TM,Hur EM,Lee BD

Tue Feb 13 00:00:00 EST 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Corallino S,Malinverno C,Neumann B,Tischer C,Palamidessi A,Frittoli E,Panagiotakopoulou M,Disanza A,Malet-Engra G,Nastaly P,Galli C,Luise C,Bertalot G,Pece S,Di Fiore PP,Gauthier N,Ferrari A,Maiuri P,Scita G

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.

The Journal of biological chemistry

Biesemann A,Gorontzi A,Barr F,Gerke V

Fri Jul 14 00:00:00 EDT 2017

Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.

The Journal of biological chemistry

Biesemann A,Gorontzi A,Barr F,Gerke V

Fri Jul 14 00:00:00 EDT 2017

Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.

Nature communications

Takasugi M,Okada R,Takahashi A,Virya Chen D,Watanabe S,Hara E

Tue Jun 06 00:00:00 EDT 2017

Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.

Nature communications

Takasugi M,Okada R,Takahashi A,Virya Chen D,Watanabe S,Hara E

Tue Jun 06 00:00:00 EDT 2017

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Histochemistry and cell biology

Wang HH,Cui Q,Zhang T,Wang ZB,Ouyang YC,Shen W,Ma JY,Schatten H,Sun QY

Wed Jun 01 00:00:00 EDT 2016

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Histochemistry and cell biology

Wang HH,Cui Q,Zhang T,Wang ZB,Ouyang YC,Shen W,Ma JY,Schatten H,Sun QY

Wed Jun 01 00:00:00 EDT 2016

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Identification of an oncogenic RAB protein.

Science (New York, N.Y.)

Wheeler DB,Zoncu R,Root DE,Sabatini DM,Sawyers CL

Fri Oct 09 00:00:00 EDT 2015

Identification of an oncogenic RAB protein.

Science (New York, N.Y.)

Wheeler DB,Zoncu R,Root DE,Sabatini DM,Sawyers CL

Fri Oct 09 00:00:00 EDT 2015

Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.

Traffic (Copenhagen, Denmark)

Dutta D,Donaldson JG

Tue Sep 01 00:00:00 EDT 2015

Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.

Traffic (Copenhagen, Denmark)

Dutta D,Donaldson JG

Tue Sep 01 00:00:00 EDT 2015

Novel functions for the endocytic regulatory proteins MICAL-L1 and EHD1 in mitosis.

Traffic (Copenhagen, Denmark)

Reinecke JB,Katafiasz D,Naslavsky N,Caplan S

Thu Jan 01 00:00:00 EST 2015

Novel functions for the endocytic regulatory proteins MICAL-L1 and EHD1 in mitosis.

Traffic (Copenhagen, Denmark)

Reinecke JB,Katafiasz D,Naslavsky N,Caplan S

Thu Jan 01 00:00:00 EST 2015

CLIC4 regulates cell adhesion and β1 integrin trafficking.

Journal of cell science

Argenzio E,Margadant C,Leyton-Puig D,Janssen H,Jalink K,Sonnenberg A,Moolenaar WH

Mon Dec 15 00:00:00 EST 2014

CLIC4 regulates cell adhesion and β1 integrin trafficking.

Journal of cell science

Argenzio E,Margadant C,Leyton-Puig D,Janssen H,Jalink K,Sonnenberg A,Moolenaar WH

Mon Dec 15 00:00:00 EST 2014

Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.

Molecular biology of the cell

Miyamoto Y,Yamamori N,Torii T,Tanoue A,Yamauchi J

Thu May 01 00:00:00 EDT 2014

Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.

Molecular biology of the cell

Miyamoto Y,Yamamori N,Torii T,Tanoue A,Yamauchi J

Thu May 01 00:00:00 EDT 2014

Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.

PLoS biology

Frühbeis C,Fröhlich D,Kuo WP,Amphornrat J,Thilemann S,Saab AS,Kirchhoff F,Möbius W,Goebbels S,Nave KA,Schneider A,Simons M,Klugmann M,Trotter J,Krämer-Albers EM

Mon Jul 01 00:00:00 EDT 2013

Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.

PLoS biology

Frühbeis C,Fröhlich D,Kuo WP,Amphornrat J,Thilemann S,Saab AS,Kirchhoff F,Möbius W,Goebbels S,Nave KA,Schneider A,Simons M,Klugmann M,Trotter J,Krämer-Albers EM

Mon Jul 01 00:00:00 EDT 2013

Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.

Cellular signalling