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RAB5 Antibody (MA5-35161) in WB
Western blot was performed using Anti-RAB5 Recombinant Rabbit Monoclonal Antibody (ARC0297) (Product # MA5-35161) and a 25 kDa band corresponding to RAB5A was observed across across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), Jurkat (Lane 3), U-87 MG (Lane 4), PC-12 (Lane 5), MCF7 (Lane 6), C2C12 (Lane 7), THP-1 (Lane 8), RAW 264.7 (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were t... View More
Product Details
MA5-35161
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0297
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 116-215 of human RAB5AA (P20339)
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849066
Product Specific Information
Immunogen sequence: KELQRQASPN IVIALSGNKA DLANKRAVDF QEAQSYADDN SLLFMETSAK TSMNVNEIFM AIAKKLPKNE PQNPGANSAR GRGVDLTEPT QPTRNQCCSN
Target Information
RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A; Small GTP-binding protein rab5
Gene Aliases: 2410015H04Rik; AI663973; AU021172; nnyRab5a; RAB5; RAB5A
UniProt ID: (Human) P20339, (Mouse) Q9CQD1, (Rat) M0RC99
Entrez Gene ID: (Human) 5868, (Mouse) 271457, (Rat) 64633
endocytosis
phagocytosis
small GTPase mediated signal transduction
blood coagulation
protein transport
regulation of endocytosis
synaptic vesicle recycling
early endosome to late endosome transport
positive regulation of exocytosis
regulation of endosome size
regulation of filopodium assembly
receptor internalization involved in canonical Wnt signaling pathway
regulation of synaptic vesicle exocytosis
regulation of autophagosome assembly
transport
intracellular protein transport
endosome organization
positive regulation of smooth muscle cell migration
Rab protein signal transduction
regulation of long-term neuronal synaptic plasticity
positive regulation of smooth muscle cell proliferation
vesicle-mediated transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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