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Invitrogen
RAB7A Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
RAB7A Antibody (PA5-78238) in ICC/IF
Immunofluorescence analysis of Ras-related protein Rab-7a was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB7A Polyclonal Antibody (Product # PA5-78238) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More
Product Details
PA5-78238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Rab7. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736494
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Xenopus laevis (100%), Dog (100%), Sheep (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein BRL-RAS; Ras-related protein p23; Ras-related protein Rab-7a
Gene Aliases: PRO2706; RAB7; RAB7A
UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527
Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448
protein targeting to lysosome
transport
intracellular protein transport
epidermal growth factor catabolic process
small GTPase mediated signal transduction
endosome to lysosome transport
protein transport
viral release from host cell
protein to membrane docking
Rab protein signal transduction
retrograde transport, endosome to Golgi
early endosome to late endosome transport
bone resorption
positive regulation of protein catabolic process
positive regulation of viral process
phagosome acidification
phagosome-lysosome fusion
positive regulation of exosomal secretion
regulation of autophagosome assembly
endocytosis
antigen processing and presentation of exogenous peptide antigen via MHC class II
lipophagy
phagosome maturation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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