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FIGURE: 1 / 1
RAB9 Antibody (PA5-79907) in WB
Western blot analysis of RAB9 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: HeLa whole cell lysate, Lane 4: PC12 whole cell lysate, Lane 5: NIH whole cell lysate, Lane 6: A431 whole cell lysate, Lane 7: 293T whole cell lysate using 40-50 µg per well. Sample was incubated with RAB9 (Product # PA5-79907) at a dilution of 0.5 µg/mL.
Product Details
PA5-79907
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9 (178-193aa RSDHLIQTDTVNLHRK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747022
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rab-9A; RAB9, member RAS oncogene family; RAB9-like protein; Ras-related protein Rab-9A; SID 99
Gene Aliases: 2410064E05Rik; AI195561; RAB9; RAB9A; Sid6061p; Sid99
UniProt ID: (Human) P51151, (Mouse) Q9R0M6, (Rat) Q99P75
Entrez Gene ID: (Human) 9367, (Mouse) 56382, (Rat) 84589
small GTPase mediated signal transduction
protein transport
regulation of protein localization
retrograde transport, endosome to Golgi
positive regulation of exocytosis
negative regulation by host of symbiont molecular function
intracellular protein transport
metabolic process
Rab protein signal transduction
phagosome-lysosome fusion
transport
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