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RAB9 Antibody (PA5-95339) in ICC/IF
Immunocytochemistry analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (Product # PA5-95339) . Rab9/RAB9A was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-Rab9/RAB9A antibody (Product # PA5-95339) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Th... View More
Product Details
PA5-95339
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9 (156-193aa KDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807142
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rab-9A; RAB9, member RAS oncogene family; RAB9-like protein; Ras-related protein Rab-9A; SID 99
Gene Aliases: 2410064E05Rik; AI195561; RAB9; RAB9A; Sid6061p; Sid99
UniProt ID: (Human) P51151, (Mouse) Q9R0M6
Entrez Gene ID: (Human) 9367, (Mouse) 56382
small GTPase mediated signal transduction
protein transport
regulation of protein localization
retrograde transport, endosome to Golgi
positive regulation of exocytosis
negative regulation by host of symbiont molecular function
transport
intracellular protein transport
metabolic process
Rab protein signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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