Proteintech

RAC1/2/3 Polyclonal Antibody

RAC1/2/3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

RAC1/2/3 Antibody (16117-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using RAC3 antibody 16117-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

RAC1/2/3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1/2/3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16117-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAC1/2/3 Fusion Protein Ag9045 (1-192 aa encoded by BC015197)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize Rac1, Rac2, and Rac3.

Immunogen sequence: MQAIKCVVV GDGAVGKTCL LISYTTNAFP GEYIPTVFDN YSANVMVDGK PVNLGLWDTA GQEDYDRLRP LSYPQTDVFL ICFSLVSPAS FENVRAKWYP EVRHHCPHTP ILLVGTKLDL RDDKDTIERL RDKKLAPITY PQGLAMAREI GSVKYLECSA LTQRGLKTVF DEAIRAVLCP PPVKKPGKKC TVF (1-192 aa encoded by BC015197)

Target Information

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cancer-associated fibroblasts promote migration and invasion of non-small cell lung cancer cells via METTL3-mediated RAC3 m⁶A modification.

International journal of biological sciences

Chen M,Zhang Q,Zheng S,Guo X,Cao L,Ren Y,Qian Y,Wang M,Wu X,Xu K

Tue Apr 18 00:00:00 EDT 2023

Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.

Glycoconjugate journal

Di Biase E,Lunghi G,Fazzari M,Maggioni M,Pomè DY,Valsecchi M,Samarani M,Fato P,Ciampa MG,Prioni S,Mauri L,Sonnino S,Chiricozzi E

Mon Jun 01 00:00:00 EDT 2020

Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.

Molecular biology of the cell

Rosenberg BJ,Gil-Henn H,Mader CC,Halo T,Yin T,Condeelis J,Machida K,Wu YI,Koleske AJ

Mon May 15 00:00:00 EDT 2017

Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.

Molecular biology of the cell

Rosenberg BJ,Gil-Henn H,Mader CC,Halo T,Yin T,Condeelis J,Machida K,Wu YI,Koleske AJ

Mon May 15 00:00:00 EDT 2017

A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.

Frontiers in cellular neuroscience

Franchi SA,Macco R,Astro V,Tonoli D,Savino E,Valtorta F,Sala K,Botta M,de Curtis I

Wed Sep 30 00:00:00 EDT 2020

A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.

Frontiers in cellular neuroscience

Franchi SA,Macco R,Astro V,Tonoli D,Savino E,Valtorta F,Sala K,Botta M,de Curtis I

Wed Sep 30 00:00:00 EDT 2020

Identification of a Protein Network Driving Neuritogenesis of MGE-Derived GABAergic Interneurons.

Frontiers in cellular neuroscience

Franchi SA,Astro V,Macco R,Tonoli D,Barnier JV,Botta M,de Curtis I

Sat Jan 29 00:00:00 EST 2022

Identification of a Protein Network Driving Neuritogenesis of MGE-Derived GABAergic Interneurons.

Frontiers in cellular neuroscience

Franchi SA,Astro V,Macco R,Tonoli D,Barnier JV,Botta M,de Curtis I

Sat Jan 29 00:00:00 EST 2022

Silencing of Rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.

Asian Pacific journal of cancer prevention : APJCP

Liu TQ,Wang GB,Li ZJ,Tong XD,Liu HX

Mon Dec 21 00:00:00 EST 2015

Silencing of Rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.

Asian Pacific journal of cancer prevention : APJCP

Liu TQ,Wang GB,Li ZJ,Tong XD,Liu HX

Mon Dec 21 00:00:00 EST 2015

F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.

Molecular cancer

Dong S,Zhao J,Wei J,Bowser RK,Khoo A,Liu Z,Luketich JD,Pennathur A,Ma H,Zhao Y

Tue Apr 01 00:00:00 EDT 2014

F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.

Molecular cancer

Dong S,Zhao J,Wei J,Bowser RK,Khoo A,Liu Z,Luketich JD,Pennathur A,Ma H,Zhao Y

Tue Apr 01 00:00:00 EDT 2014

Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.

BMC cancer

Gest C,Joimel U,Huang L,Pritchard LL,Petit A,Dulong C,Buquet C,Hu CQ,Mirshahi P,Laurent M,Fauvel-Lafève F,Cazin L,Vannier JP,Lu H,Soria J,Li H,Varin R,Soria C

Wed Feb 06 00:00:00 EST 2013

Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.

BMC cancer

Gest C,Joimel U,Huang L,Pritchard LL,Petit A,Dulong C,Buquet C,Hu CQ,Mirshahi P,Laurent M,Fauvel-Lafève F,Cazin L,Vannier JP,Lu H,Soria J,Li H,Varin R,Soria C

Wed Feb 06 00:00:00 EST 2013

Bioinformatics

Protein Aliases: Cell migration-inducing gene 5 protein; EN-7 protein; GX; p21-Rac1; p21-Rac2; p21-Rac3; Protein EN-7; Ras-like protein TC25; RAS-related C3 botulinum substrate 2; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); Ras-related C3 botulinum toxin substrate 2; Ras-related C3 botulinum toxin substrate 3; ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac3; Small G protein

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Gene Aliases: AI323801; AI452260; AL023026; D5Ertd559e; EN-7; Gx; HSPC022; MIG5; p21-Rac1; p21-Rac2; Rac; Rac-1; RAC1; Rac1B; RAC2; RAC3; TC-25; TC25

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UniProt ID: (Human) P63000, (Human) P15153, (Human) P60763, (Mouse) P63001, (Rat) Q6RUV5, (Mouse) Q05144, (Mouse) P60764

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Entrez Gene ID: (Human) 5879, (Human) 5880, (Human) 5881, (Mouse) 19353, (Rat) 363875, (Rat) 366957, (Mouse) 19354, (Mouse) 170758, (Rat) 688319

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Function(s)

GTPase activity protein binding GTP binding Rab GTPase binding enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding histone deacetylase binding Rho GDP-dissociation inhibitor binding protein kinase regulator activity calcium-dependent protein binding nucleotide binding small GTPase immunoglobulin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAC1/2/3 Polyclonal Antibody

RAC1/2/3 Antibody (16117-1-AP) in ICC/IF

Product Details

16117-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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