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RAC2 Antibody (PA5-102362) in ICC/IF
Immunocytochemistry analysis of RAC2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAC2 polyclonal antibody (Product # PA5-102362) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102362
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAC2(Accession P15153), corresponding to amino acid residues L160-L192.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851769
Product Specific Information
Antibody detects endogenous levels of total RAC2.
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42 which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EN-7 protein; GX; p21-Rac2; Protein EN-7; RAS-related C3 botulinum substrate 2; Ras-related C3 botulinum toxin substrate 2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); Small G protein
Gene Aliases: AI323801; AI452260; EN-7; Gx; HSPC022; p21-Rac2; RAC2
UniProt ID: (Human) P15153, (Mouse) Q05144
Entrez Gene ID: (Human) 5880, (Rat) 366957, (Mouse) 19354
chemotaxis
actin filament organization
signal transduction
G-protein coupled receptor signaling pathway
small GTPase mediated signal transduction
positive regulation of cell proliferation
regulation of hydrogen peroxide metabolic process
positive regulation of lamellipodium assembly
regulation of cell-substrate adhesion
cell projection assembly
platelet activation
regulation of T cell proliferation
regulation of mast cell degranulation
bone resorption
regulation of protein kinase activity
regulation of small GTPase mediated signal transduction
regulation of respiratory burst
regulation of mast cell chemotaxis
lymphocyte aggregation
positive regulation of neutrophil chemotaxis
regulation of neutrophil migration
positive regulation of protein targeting to mitochondrion
metabolic process
actin cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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