Proteintech

RAD1 Polyclonal Antibody

RAD1 Antibody in Immunocytochemistry (ICC/IF)

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RAD1 Antibody (11726-2-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11726-2-AP (RAD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11726-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fish, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAD1 Fusion Protein Ag2316 (1-282 aa encoded by BC006837)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPLLTQQIQ DEDDQYSLVA SLDNVRNLST ILKAIHFREH ATCFATKNGI KVTVENAKCV QANAFIQAGI FQEFKVQEES VTFRINLTVL LDCLSIFGSS PMPGTLTALR MCYQGYGYPL MLFLEEGGVV TVCKINTQEP EETLDFDFCS TNVINKIILQ SEGLREAFSE LDMTSEVLQI TMSPDKPYFR LSTFGNAGSS HLDYPKDSDL MEAFHCNQTQ VNRYKISLLK PSTKALVLSC KVSIRTDNRG FLSLQYMIRN EDGQICFVEY YCCPDEEVPE SES (1-282 aa encoded by BC006837)

Target Information

Rad1 is a component of a heterotrimeric PCNA-like complex that contains Rad9 and Hus1 proteins. This complex is involved in the 9-1-1 cell cycle checkpoing response complex that plays a major role in DNA repair, by associating with Rad17 and several components of the PCNA-loading heteropentamer replication factor C. Mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia.

Molecular medicine (Cambridge, Mass.)

Tong X,Li D,Liu N,Huang W,Zhao X,Zhang D,Xue X,Fu J

Wed May 24 00:00:00 EDT 2023

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Oncogene

Chen B,Xu F,Gao Y,Hu G,Zhu K,Lu H,Xu A,Chen S,Wu L,Zhao G

Tue Mar 01 00:00:00 EST 2022

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

American journal of physiology. Cell physiology

Shi R,Wang Y,Gao Y,Xu X,Mao S,Xiao Y,Song S,Wang L,Tian B,Zhao Y,Hua Y,Xu H

Thu Oct 01 00:00:00 EDT 2020

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Journal of molecular cell biology

Xu X,Shi R,Zheng L,Guo Z,Wang L,Zhou M,Zhao Y,Tian B,Truong K,Chen Y,Shen B,Hua Y,Xu H

Mon Oct 01 00:00:00 EDT 2018

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Journal of molecular cell biology

Xu X,Shi R,Zheng L,Guo Z,Wang L,Zhou M,Zhao Y,Tian B,Truong K,Chen Y,Shen B,Hua Y,Xu H

Mon Oct 01 00:00:00 EDT 2018

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Bozdarov J,Sherry JP,Duncker BP,Bols NC,Dixon B

Tue Jan 01 00:00:00 EST 2013

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Bozdarov J,Sherry JP,Duncker BP,Bols NC,Dixon B

Tue Jan 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein RAD1; checkpoint control protein HRAD1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; exonuclease homolog RAD1; hRAD1; mRAD1; RAD1 checkpoint clamp component; RAD1 homolog; Rad1-like DNA damage checkpoint protein

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Gene Aliases: HRAD1; mRAD1; RAD1; REC1

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UniProt ID: (Human) O60671, (Mouse) Q9QWZ1

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Entrez Gene ID: (Human) 5810, (Rat) 294800, (Mouse) 19355

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Function(s)

damaged DNA binding protein binding 3'-5' exonuclease activity exodeoxyribonuclease III activity nuclease activity exonuclease activity hydrolase activity DNA metabolism protein exodeoxyribonuclease nucleic acid metabolism protein

Process(es)

DNA damage checkpoint DNA replication DNA repair cellular response to DNA damage stimulus substantia nigra development meiotic recombination checkpoint cellular response to ionizing radiation nucleic acid phosphodiester bond hydrolysis regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAD1 Polyclonal Antibody

RAD1 Antibody (11726-2-AP) in ICC/IF

Product Details

11726-2-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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