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RAD18 Antibody (PA5-22320) in ICC/IF
RAD18 Polyclonal Antibody detects RAD18 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD18 protein stained by RAD18 Polyclonal Antibody (Product # PA5-22320) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-22320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD18 (Uniprot ID#Q9NS91)
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11154266
Product Specific Information
Predicted reactivity: Pig (83%), Bovine (80%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD18 E3 ubiquitin protein ligase; RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18
Gene Aliases: RAD18; RNF73
UniProt ID: (Human) Q9NS91
Entrez Gene ID: (Human) 56852
Y-form DNA binding
damaged DNA binding
protein binding
zinc ion binding
ligase activity
polyubiquitin binding
ubiquitin protein ligase binding
protein complex binding
identical protein binding
single-stranded DNA-dependent ATPase activity
ubiquitin protein ligase activity
ubiquitin-protein ligase
protein modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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