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RAD21 Antibody (PA5-109313) in ICC/IF
Immunofluorescent analysis of Rad21 in SK-Br-3 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Rad21 polyclonal antibody (Product # PA5-109313) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Product Details
PA5-109313
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human Rad21 aa 252-456
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854724
Product Specific Information
Positive Control: Mouse ovary tissue, Daudi, MG-63, SiHa, SK-Br-3, rat brain tissue, human thyroid gland cancer tissue, human colon tissue, mouse testis tissue.
Target Information
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double-strand-break repair protein rad21 homolog; FLJ25655; FLJ40596; h NXP-1; hHR21; KIAA0078RAD21; kleisin; Nuclear matrix protein 1; NXP-1; protein involved in DNA double-strand break repair; RAD21 homolog; SCC1 homolog; sister chromatid cohesion 1
Gene Aliases: CDLS4; hHR21; HR21; HRAD21; KIAA0078; MCD1; NXP1; RAD21; SCC1
UniProt ID: (Human) O60216
Entrez Gene ID: (Human) 5885
double-strand break repair
DNA recombination
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
sister chromatid cohesion
mitotic nuclear division
reciprocal meiotic recombination
protein sumoylation
positive regulation of transcription from RNA polymerase II promoter
cell division
protein localization to chromatin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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