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RAD21 Antibody (PA5-70233) in WB
Western blot using RAD21 Polyclonal Antibody (Product # PA5-70233) and a band at ~90 kDa corresponding to RAD21 was decreased upon etoposide treatment in HeLa. Few uncharacterized bands (*) were also observed at lower molecular weights. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with 50µM Etoposide for 48 hrs (Lane 2), were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More
Product Details
PA5-70233
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide corresponding to a region of rat RAD21
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2691048
Product Specific Information
This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Target Information
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: double-strand-break repair protein rad21 homolog; FLJ25655; FLJ40596; h NXP-1; KIAA0078RAD21
Entrez Gene ID: (Rat) 314949
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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