Invitrogen

RAD51 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

22 References

View all (42) RAD51 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RAD51 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

RAD51 Antibody (PA5-27195) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of RAD51 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD51 Polyclonal Antibody (Product # PA5-27195) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAD51 Antibody in Immunoprecipitation (IP)

RAD51 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC)

RAD51 Antibody in Immunohistochemistry (IHC)

RAD51 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RAD51 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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RAD51 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with RAD51 shRNA and decrease in signal intensity was observed in Western blot application using RAD51 Polyclonal Antibody (Product # PA5-27195). {KD}

Product Details

PA5-27195

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 10 publications 10 publications

Immunoprecipitation (IP)

1:100-1:500

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 228 of Human Rad51

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.95 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544671

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2.

Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (90%), Xenopus laevis (96%), Dog (98%), Pig (99%), Rabbit (97%), Chicken (95%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development.

The Journal of biological chemistry

Zhao S,Huang C,Yang Y,Xu W,Yu Y,Wen C,Cao L,Gao F,Qin Y,Chen ZJ,Guo T,Zhao S

PA5-27195 was used in Immunohistochemistry-immunofluorescence to suggest that FANCD2 has distinct functions that are both dependent on and independent of its ubiquitination during germ cell development.

Wed Mar 01 00:00:00 EST 2023

LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.

Nucleic acids research

Yu R,Hu Y,Zhang S,Li X,Tang M,Yang M,Wu X,Li Z,Liao X,Xu Y,Li M,Chen S,Qian W,Gong LY,Song L,Li J

PA5-27195 was used in Immunocytochemistry-immunofluorescence to find that DNA damage provoked the association of ribosomes with IRES region in lncRNA CTBP1-DT, which overcame the negative effect of upstream open reading frames (uORFs), and elicited the novel microprotein DNA damage-upregulated protein (DDUP) translation via a cap-independent translation mechanism.

Fri Aug 12 00:00:00 EDT 2022

RAD51 is essential for spermatogenesis and male fertility in mice.

Cell death discovery

Qin J,Huang T,Wang J,Xu L,Dang Q,Xu X,Liu H,Liu Z,Shao C,Zhang X

Published figure using RAD51 polyclonal antibody (Product # PA5-27195) in Immunocytochemistry

Tue Mar 15 00:00:00 EDT 2022

RAD51 is essential for spermatogenesis and male fertility in mice.

Cell death discovery

Qin J,Huang T,Wang J,Xu L,Dang Q,Xu X,Liu H,Liu Z,Shao C,Zhang X

Published figure using RAD51 polyclonal antibody (Product # PA5-27195) in Immunocytochemistry

Tue Mar 15 00:00:00 EDT 2022

RAD51 is essential for spermatogenesis and male fertility in mice.

Cell death discovery

Qin J,Huang T,Wang J,Xu L,Dang Q,Xu X,Liu H,Liu Z,Shao C,Zhang X

Published figure using RAD51 polyclonal antibody (Product # PA5-27195) in Immunocytochemistry

Tue Mar 15 00:00:00 EDT 2022

Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.

Scientific reports

Tesfay L,Paul BT,Hegde P,Brewer M,Habbani S,Jellison E,Moore T,Wu H,Torti SV,Torti FM

Published figure using RAD51 polyclonal antibody (Product # PA5-27195) in Immunocytochemistry

Sat Nov 19 00:00:00 EST 2022

MEIOK21 regulates oocyte quantity and quality via modulating meiotic recombination.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Shang Y,Huang J,Li W,Zhang Y,Zhou X,Shao Q,Tan T,Yin S,Zhang L,Wang S

PA5-27195 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate critical roles for MEIOK21 in ensuring the number and quality of oocytes in the follicles.

Wed Jun 01 00:00:00 EDT 2022

Selinexor is a novel inhibitor of DNA damage response in Merkel cell carcinoma.

Clinical and experimental dermatology

Moore SA,Narayanan D,Simonette RA,Bartley BR,Doan HQ,Rady PL,Tyring SK

PA5-27195 was used in Gel Electrophoresis to show that Selinexor inhibited DDR protein expression in both MCPyV-positive and MCPyV-negative cells, so addition of Selinexor alone or combined with ICI may be a promising treatment for MCC.

Fri Jul 01 00:00:00 EDT 2022

Gut Microbiota Dysbiosis Accelerates Prostate Cancer Progression Through Increased LPCAT1 Expression and Enhanced DNA Repair Pathways.

Frontiers in oncology

Liu Y,Yang C,Zhang Z,Jiang H

PA5-27195 was used in Western Blot to determine the potential mechanism of microbiota dysbiosis in prostate cancer (PCa) progression.

Tue Jul 06 00:00:00 EDT 2021

Gut Microbiota Dysbiosis Accelerates Prostate Cancer Progression Through Increased LPCAT1 Expression and Enhanced DNA Repair Pathways.

Frontiers in oncology

Liu Y,Yang C,Zhang Z,Jiang H

PA5-27195 was used in Western Blot to determine the potential mechanism of microbiota dysbiosis in prostate cancer (PCa) progression.

Tue Jul 06 00:00:00 EDT 2021

Gut Microbiota Dysbiosis Accelerates Prostate Cancer Progression Through Increased LPCAT1 Expression and Enhanced DNA Repair Pathways.

Frontiers in oncology

Liu Y,Yang C,Zhang Z,Jiang H

PA5-27195 was used in Western Blot to determine the potential mechanism of microbiota dysbiosis in prostate cancer (PCa) progression.

Tue Jul 06 00:00:00 EDT 2021

Chloroxine overrides DNA damage tolerance to restore platinum sensitivity in high-grade serous ovarian cancer.

Cell death & disease

Silva VL,Saxena J,Nicolini F,Hoare JI,Metcalf S,Martin SA,Lockley M

PA5-27195 was used in Immunocytochemistry, Western Blot to investigate the activity of chloroxine as an anticancer agent and its cellular mechanism as well as its synergy with platinum agents in resistant high-grade serous cancer both in vitro and in vivo.

Wed Apr 14 00:00:00 EDT 2021

Chloroxine overrides DNA damage tolerance to restore platinum sensitivity in high-grade serous ovarian cancer.

Cell death & disease

Silva VL,Saxena J,Nicolini F,Hoare JI,Metcalf S,Martin SA,Lockley M

Wed Apr 14 00:00:00 EDT 2021

Chloroxine overrides DNA damage tolerance to restore platinum sensitivity in high-grade serous ovarian cancer.

Cell death & disease

Silva VL,Saxena J,Nicolini F,Hoare JI,Metcalf S,Martin SA,Lockley M

Wed Apr 14 00:00:00 EDT 2021

Chloroxine overrides DNA damage tolerance to restore platinum sensitivity in high-grade serous ovarian cancer.

Cell death & disease

Silva VL,Saxena J,Nicolini F,Hoare JI,Metcalf S,Martin SA,Lockley M

Wed Apr 14 00:00:00 EDT 2021

CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment.

Cell reports

Klomp JE,Lee YS,Goodwin CM,Papke B,Klomp JA,Waters AM,Stalnecker CA,DeLiberty JM,Drizyte-Miller K,Yang R,Diehl JN,Yin HH,Pierobon M,Baldelli E,Ryan MB,Li S,Peterson J,Smith AR,Neal JT,McCormick AK,Kuo CJ,Counter CM,Petricoin EF,Cox AD,Bryant KL,Der CJ

PA5-27195 was used in Immunocytochemistry-immunoflourescence to conclude that concurrent DDR suppression enhances the efficacy of ERK and/or autophagy inhibitors in KRAS mutant PDAC.

Tue Nov 30 00:00:00 EST 2021

A phase I dose-finding, pharmacokinetics and genotyping study of olaparib and lurbinectedin in patients with advanced solid tumors.

Scientific reports

Poveda A,Oaknin A,Romero I,Guerrero-Zotano A,Fariñas-Madrid L,Rodriguez-Freixinos V,Mallol P,Lopez-Reig R,Lopez-Guerrero JA

PA5-27195 was used in Western Blotting to determine recommended, dose-limiting toxicities, and maximum tolerated doses of anti-cancer drugs in ovarian and breast cancer.

Wed Feb 24 00:00:00 EST 2021

MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.

Nucleic acids research

Shang Y,Huang T,Liu H,Liu Y,Liang H,Yu X,Li M,Zhai B,Yang X,Wei Y,Wang G,Chen Z,Wang S,Zhang L

PA5-27195 was used in Immunohistochemistry to investigate if MEIOK21 is a component of meiotic recombination bridges and thus required for efficient formation of DMC1/RAD51 foci.

Thu Jul 09 00:00:00 EDT 2020

MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.

Nucleic acids research

Shang Y,Huang T,Liu H,Liu Y,Liang H,Yu X,Li M,Zhai B,Yang X,Wei Y,Wang G,Chen Z,Wang S,Zhang L

PA5-27195 was used in Immunohistochemistry to investigate if MEIOK21 is a component of meiotic recombination bridges and thus required for efficient formation of DMC1/RAD51 foci.

Thu Jul 09 00:00:00 EDT 2020

The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.

Nature communications

Zhang J,Gurusaran M,Fujiwara Y,Zhang K,Echbarthi M,Vorontsov E,Guo R,Pendlebury DF,Alam I,Livera G,Emmanuelle M,Wang PJ,Nandakumar J,Davies OR,Shibuya H

PA5-27195 was used in Immunocytochemistry-immunoflourescence to identify BRCA2 and MEILB2-associating protein 1 (BRME1), which functions as a stabilizer of MEILB2 by binding to an α-helical N-terminus of MEILB2 and preventing MEILB2 self-association.

Tue Apr 28 00:00:00 EDT 2020

The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.

Nature communications

Zhang J,Gurusaran M,Fujiwara Y,Zhang K,Echbarthi M,Vorontsov E,Guo R,Pendlebury DF,Alam I,Livera G,Emmanuelle M,Wang PJ,Nandakumar J,Davies OR,Shibuya H

Tue Apr 28 00:00:00 EDT 2020

The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.

Nature communications

Zhang J,Gurusaran M,Fujiwara Y,Zhang K,Echbarthi M,Vorontsov E,Guo R,Pendlebury DF,Alam I,Livera G,Emmanuelle M,Wang PJ,Nandakumar J,Davies OR,Shibuya H

Tue Apr 28 00:00:00 EDT 2020

The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair.

eLife

Huang T,Yuan S,Gao L,Li M,Yu X,Zhan J,Yin Y,Liu C,Zhang C,Lu G,Li W,Liu J,Chen ZJ,Liu H

PA5-27195 was used in Immunocytochemistry to demonstrate the function of ZCWPW1 that acts as part of the selection system for epigenetics-based recombination hotspots in mammals.

Wed May 06 00:00:00 EDT 2020

SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice.

Nucleic acids research

Liu H,Huang T,Li M,Li M,Zhang C,Jiang J,Yu X,Yin Y,Zhang F,Lu G,Luo MC,Zhang LR,Li J,Liu K,Chen ZJ

PA5-27195 was used in Immunocytochemistry-immunoflourescence to identify a novel protein, synaptonemal complex reinforcing element as a key molecule in maintaining the integrity of synaptonemal complex during meiosis prophase 1 in mice.

Thu Jun 20 00:00:00 EDT 2019

A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.

Nature communications

Zhang J,Fujiwara Y,Yamamoto S,Shibuya H

PA5-27195 was used in Western Blotting to study the role of BRCA2 in the repair mechanism of double stranded breaks.

Wed Feb 13 00:00:00 EST 2019

A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.

Nature communications

Zhang J,Fujiwara Y,Yamamoto S,Shibuya H

PA5-27195 was used in Western Blotting to study the role of BRCA2 in the repair mechanism of double stranded breaks.

Wed Feb 13 00:00:00 EST 2019

Drugging DNA repair to target T-ALL cells.

Leukemia & lymphoma

Dasgupta Y,Golovine K,Nieborowska-Skorska M,Luo L,Matlawska-Wasowska K,Mullighan CG,Skorski T

PA5-27195 was used in Immunocytochemistry to hypothesize that targeting PARP1 and RAD52 will induce synthetic lethality in TALLs/ETP-ALLs displaying 'BRCAness' and spare normal cells.

Sun Jul 01 00:00:00 EDT 2018

Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast.

Nucleic acids research

Manjón E,Edreira T,Muñoz S,Sánchez Y

PA5-27195 was used in Immunohistochemistry-immunofluorescence to explore the sensitivity of a deletion mutation in the Rho1p exchange factor Rgf1p to the DNA break/inducing antibiotic phleomycin.

Fri May 19 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

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Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603; wu:fb38e12; zgc:77754

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UniProt ID: (Human) Q06609, (Mouse) Q08297

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Entrez Gene ID: (Human) 5888, (Mouse) 19361, (Rat) 499870, (Zebrafish) 406487

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Function(s)

recombinase activity four-way junction DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding protein C-terminus binding identical protein binding single-stranded DNA-dependent ATPase activity DNA polymerase binding nucleotide binding DNA binding damaged DNA binding DNA-dependent ATPase activity molecular_function DNA metabolism protein

Process(es)

telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA synthesis involved in DNA repair strand displacement regulation of protein phosphorylation DNA unwinding involved in DNA replication DNA repair DNA recombination mitotic recombination cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening replication fork processing strand invasion response to drug positive regulation of DNA ligation protein homooligomerization chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to gamma radiation cellular response to hydroxyurea cellular response to cisplatin cellular response to camptothecin response to etoposide replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing DNA metabolic process telomere organization cellular response to alkaloid response to ionizing radiation response to cadmium ion response to methylmercury hematopoietic stem cell proliferation

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RAD51 Polyclonal Antibody

RAD51 Antibody (PA5-27195) in ICC/IF

Product Details

PA5-27195

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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