Invitrogen

RAD51 Polyclonal Antibody

2 Published Figures

4 References

View all (42) RAD51 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

FIGURE: 1 / 3

RAD51 Antibody (PA5-34905) in WB

Western Blot using RAD51 Polyclonal Antibody (Product # PA5-34905). Sample (10 µg of whole cell lysate). Lane A: yeast . 7.5% SDS PAGE. RAD51 Polyclonal Antibody (Product # PA5-34905) diluted at 1:1,000.

Product Image Gallery

Product Details

PA5-34905

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD51 (Uniprot ID#P25454)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552256

Product Specific Information

Recommended positive controls: yeast.

Predicted reactivity: Yeast (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Structure-forming CAG/CTG repeats interfere with gap repair to cause repeat expansions and chromosome breaks.

Nature communications

Polleys EJ,Del Priore I,Haber JE,Freudenreich CH

PA5-34905 was used in ChIP Assay to develop an assay such that resection and ssDNA gap fill-in would occur across a (CAG)70 or (CTG)70 repeat tract.

Sat Apr 29 00:00:00 EDT 2023

Rad9/53BP1 promotes DNA repair via crossover recombination by limiting the Sgs1 and Mph1 helicases.

Nature communications

Ferrari M,Rawal CC,Lodovichi S,Vietri MY,Pellicioli A

PA5-34905 was used in Chromatin immunoprecipitation to shed light on how cells couple DNA damage checkpoint (DDC) with the choice and effectiveness of homologous recombination (HR) sub-pathways, with implications for genome instability and cancer.

Tue Jun 23 00:00:00 EDT 2020

Rad9/53BP1 promotes DNA repair via crossover recombination by limiting the Sgs1 and Mph1 helicases.

Nature communications

Ferrari M,Rawal CC,Lodovichi S,Vietri MY,Pellicioli A

Tue Jun 23 00:00:00 EDT 2020

Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism.

PLoS genetics

Jedrychowska M,Denkiewicz-Kruk M,Alabrudzinska M,Skoneczna A,Jonczyk P,Dmowski M,Fijalkowska IJ

PA5-34905 was used in Western Blotting to propose that defective functioning of the CMG-E complex in psf1-1 cells impairs the progression of DNA replication what increases the contribution of repair mechanisms such as template switch and break-induced replication.

Sun Dec 01 00:00:00 EST 2019

Lack of G1/S control destabilizes the yeast genome via replication stress-induced DSBs and illegitimate recombination.

Journal of cell science

Krol K,Antoniuk-Majchrzak J,Skoneczny M,Sienko M,Jendrysek J,Rumienczyk I,Halas A,Kurlandzka A,Skoneczna A

PA5-34905 was used in Western Blotting to show that a lack of Swi6 causes replication stress leading to double-strand break formation, inefficient DNA repair and DNA content alterations, resulting in high cell mortality.

Mon Dec 17 00:00:00 EST 2018

Bioinformatics

Protein Aliases: DNA repair protein RAD51

View more View less

Gene Aliases: MUT5; RAD51; YER095W

View more View less

UniProt ID: (Baker's yeast) P25454

View more View less

Entrez Gene ID: (Baker's yeast) 856831

View more View less

Function(s)

recombinase activity nucleotide binding DNA binding double-stranded DNA binding single-stranded DNA binding protein binding ATP binding DNA-dependent ATPase activity ATP-dependent DNA damage sensor activity DNA metabolism protein

Process(es)

meiotic joint molecule formation telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA metabolic process DNA repair nucleotide-excision repair double-strand break repair DNA recombination mitotic recombination cellular response to DNA damage stimulus reciprocal meiotic recombination heteroduplex formation strand invasion mitochondrial DNA repair chromosome organization involved in meiotic cell cycle mitotic recombination-dependent replication fork processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RAD51 Polyclonal Antibody

RAD51 Antibody (PA5-34905) in WB

Product Details

PA5-34905

Western Blot (WB)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!