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Product Details
5888-MSM6-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
RAD51, 2856
Immunogen
Recombinant fragment of human RAD51 protein (around aa 1-134)
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA
UniProt ID: (Human) Q06609
Entrez Gene ID: (Human) 5888
recombinase activity
four-way junction DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
ATP binding
protein C-terminus binding
identical protein binding
single-stranded DNA-dependent ATPase activity
DNA polymerase binding
DNA metabolism protein
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA synthesis involved in DNA repair
strand displacement
regulation of protein phosphorylation
DNA unwinding involved in DNA replication
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
strand invasion
response to drug
positive regulation of DNA ligation
protein homooligomerization
chromosome organization involved in meiotic cell cycle
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to hydroxyurea
cellular response to cisplatin
cellular response to camptothecin
response to etoposide
replication-born double-strand break repair via sister chromatid exchange
mitotic recombination-dependent replication fork processing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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