Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
RAD54L2 Antibody (PA5-89311) in ICC/IF
Immunofluorescence analysis of RAD54L2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAD54L2 Polyclonal Antibody (Product # PA5-89311) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
Product Details
PA5-89311
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1178-1467 of human RAD54L2 (NP_0559212)
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805484
Product Specific Information
Immunogen sequence: ELQQYADVAA ARESRQSSPS TNAALPGPPA QLMDSSAVPG TALGTEPRLG GHCLNSSLLV TGQPCGDRHP VLDLRGHKRK LATPPAAQES SRRRSRKGHL PAPVQPYEHG YPVSGGFAMP PVSLNHNLTT PFTSQAGENS LFMGSTPSYY QLSNLLADAR LVFPVTTDPL VPAGPVSSSS TATSVTASNP SFMLNPSVPG ILPSYSLPFS QPLLSEPRMF APFPSPVLPS NLSRGMSIYP GYMSPHAGYP AGGLLRSQVP PFDSHEVAEV GFSSNDDEDK DDDVIEVTGK
Target Information
Adrenergic receptors (ARs) include four general types (a1, a2, b1 and b2) that are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins. ARs play a critical role in the development of prostate cancer, and transcriptional activity of AR is partly regulated by coregulatory proteins. RAD54L2 (RAD54-like 2), also known as ARIP4 (androgen receptor-interacting protein 4), HSPC325 or SRISNF2L, is a 1,467 amino acid nuclear protein belonging to the SNF2/RAD54 helicase family that consists of one helicase ATP-binding domain and a helicase C-terminal domain. RAD54L2 is a DNA helicase that regulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. RAD54L2 is post-translationally sumoylated or phosphorylated upon DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Androgen receptor-interacting protein 4; AR interacting protein 4; Helicase ARIP4; RAD54-like protein 2
Gene Aliases: ARIP4; HSPC325; KIAA0809; RAD54L2; SRISNF2L
UniProt ID: (Human) Q9Y4B4
Entrez Gene ID: (Human) 23132
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support