Invitrogen

RAGE Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

17 References

View all (26) RAGE antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RAGE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 15

RAGE Antibody (PA1-075) in IHC (P)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal... View More

RAGE Antibody in Immunohistochemistry (IHC)

RAGE Antibody in Western Blot, Neutralization (WB, Neu)

Product Image Gallery

RAGE Antibody — Figure 1. High-mobility group box1 (HMGB1) upregulated MUC5AC and MUC5B protein expression, and gene regulation was blocked by a receptor for advanced glycation end products (RAGE) inhibitor. Normal human bronchial epithelial (NHBE) cells were cultured at the air-liquid interface, treated with HMGB1 (10 or 100 ng/mL) for 24 or 48 hours, and analyzed for relative MUC5AC (A) and MUC5B (B) protein levels in the apical media by microtiter plate assay ( see the data supplement). Involvement of the RAGE receptor in HMGB1-induced MUC5AC (C) and MUC5B (D) mRNA expression was tested by pretreatment with a RAGE inhibitor, FPS-ZMI (500 nM), for 1 hour, followed by administration of HMGB1 (10 ng/mL, 24 h). Expression of MUC5AC and MUC5B mRNA was determined by qRT-PCR as described in the data supplement. HMGB1 (10 or 100 ng/mL; 24-48 h) increased AGER (RAGE) mRNA expression (E) and RAGE protein levels as shown by densitometric analysis (F) of Western blots in well-differentiated, primary NHBE cells. A representative Western blot for RAGE is shown in G. RAGE mRNA was determined by qRT-PCR, and RAGE protein levels were compared with vehicle control-treated cells by Western blot, as described in the data supplement. Data are expressed as mean +- SEM. P < 0.05 was considered statistically significant (* P < 0.05, ** P < 0.01, and *** P < 0.001 comparisons between conditions are marked by lines). Two to five independent experiments with six or more replic {TM}

Product Details

PA1-075

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1-2 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2269243

Product Specific Information

PA1-075 detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples.

PA1-075 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.

The PA1-075 immunogen is a synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. This sequence is 100% conserved in mouse and 70% conserved in humans. This peptide (Cat. # PEP-194) is available for use in neutralization and control experiments.

Target Information

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulating Morphological and Redox/Glycative Alterations in the PCOS Uterus: Effects of Carnitines in PCOS Mice.

Biomedicines

Palmerini MG,Macchiarelli G,Cocciolone D,Mascitti IA,Placidi M,Vergara T,Di Emidio G,Tatone C

Published figure using RAGE polyclonal antibody (Product # PA1-075) in Western Blot

Fri Jan 27 00:00:00 EST 2023

Modulating Morphological and Redox/Glycative Alterations in the PCOS Uterus: Effects of Carnitines in PCOS Mice.

Biomedicines

Palmerini MG,Macchiarelli G,Cocciolone D,Mascitti IA,Placidi M,Vergara T,Di Emidio G,Tatone C

Published figure using RAGE polyclonal antibody (Product # PA1-075) in Western Blot

Fri Jan 27 00:00:00 EST 2023

Metformin improves cognitive impairment in diabetic mice induced by a combination of streptozotocin and isoflurane anesthesia.

Bioengineered

Zhang W,Zhao L,Zhang J,Li P,Lv Z

Published figure using RAGE polyclonal antibody (Product # PA1-075) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Inhibition of the Receptor for Advanced Glycation End Products Enhances the Cytotoxic Effect of Gemcitabine in Murine Pancreatic Tumors.

Biomolecules

Swami P,O'Connell KA,Thiyagarajan S,Crawford A,Patil P,Radhakrishnan P,Shin S,Caffrey TC,Grunkemeyer J,Neville T,Vetter SW,Hollingsworth MA,Leclerc E

PA1-075 was used in Western Blot to demonstrate that RAGE inhibition concurrent with gemcitabine treatment enhanced the cytotoxic effect of gemcitabine.

Thu Apr 01 00:00:00 EDT 2021

Can the AGE/RAGE/ERK signalling pathway and the epithelial-to-mesenchymal transition interact in the pathogenesis of chronic rhinosinusitis with nasal polyps?

European journal of histochemistry : EJH

Vetuschi A,Pompili S,Di Marco GP,Calvaruso F,Iacomino E,Angelosante L,Festuccia C,Colapietro A,Sferra R

PA1-075 was used in Immunohistochemistry to suggest a complex network of processes in the pathogenesis of NP, and the AGE/RAGE/ERK pathway and EMT might work together in promoting tissue remodelling in the formation of CRSwNP.

Thu Jan 23 00:00:00 EST 2020

Can the AGE/RAGE/ERK signalling pathway and the epithelial-to-mesenchymal transition interact in the pathogenesis of chronic rhinosinusitis with nasal polyps?

European journal of histochemistry : EJH

Vetuschi A,Pompili S,Di Marco GP,Calvaruso F,Iacomino E,Angelosante L,Festuccia C,Colapietro A,Sferra R

Thu Jan 23 00:00:00 EST 2020

Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.

Antioxidants (Basel, Switzerland)

Di Emidio G,Rea F,Placidi M,Rossi G,Cocciolone D,Virmani A,Macchiarelli G,Palmerini MG,D'Alessandro AM,Artini PG,Tatone C

PA1-075 was used in Western Blotting to investigate whether acyl-L-carnitines improve polycystic ovary syndrome (PCOS) phenotype in a mouse model induced by dehydroepiandrosterone (DHEA).

Tue Sep 15 00:00:00 EDT 2020

Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.

Antioxidants (Basel, Switzerland)

Di Emidio G,Rea F,Placidi M,Rossi G,Cocciolone D,Virmani A,Macchiarelli G,Palmerini MG,D'Alessandro AM,Artini PG,Tatone C

PA1-075 was used in Western Blotting to investigate whether acyl-L-carnitines improve polycystic ovary syndrome (PCOS) phenotype in a mouse model induced by dehydroepiandrosterone (DHEA).

Tue Sep 15 00:00:00 EDT 2020

Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation.

Nutrients

Mastrocola R,Collotta D,Gaudioso G,Le Berre M,Cento AS,Ferreira Alves G,Chiazza F,Verta R,Bertocchi I,Manig F,Hellwig M,Fava F,Cifani C,Aragno M,Henle T,Joshi L,Tuohy K,Collino M

PA1-075 was used in Immunohistochemistry to explore the impact of an AGE-enriched diet on markers of metabolic and inflammatory disorders as well as on gut microbiota composition and plasma proteins glycosylation pattern.

Wed Aug 19 00:00:00 EDT 2020

Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation.

Nutrients

Mastrocola R,Collotta D,Gaudioso G,Le Berre M,Cento AS,Ferreira Alves G,Chiazza F,Verta R,Bertocchi I,Manig F,Hellwig M,Fava F,Cifani C,Aragno M,Henle T,Joshi L,Tuohy K,Collino M

Wed Aug 19 00:00:00 EDT 2020

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.

Cells

Emidio GD,Placidi M,Rea F,Rossi G,Falone S,Cristiano L,Nottola S,D'Alessandro AM,Amicarelli F,Palmerini MG,Tatone C

PA1-075 was used in Western Blot to show ovarian dysfunctions associated to polycystic ovary syndrome involves methylglyoxal-dependent glycative stress.

Tue Jan 14 00:00:00 EST 2020

High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells.

American journal of respiratory cell and molecular biology

Kummarapurugu AB,Zheng S,Ledford J,Karandashova S,Voynow JA

PA1-075 was used in Western Blotting to identify that high-mobility group box 1 within primary airway epithelial cells functions as an upregulator of mRNA expression and protein secretion.

Mon Jan 01 00:00:00 EST 2018

Immunolocalization of Advanced Glycation End Products, Mitogen Activated Protein Kinases, and Transforming Growth Factor-β/Smads in Pelvic Organ Prolapse.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Vetuschi A,Pompili S,Gallone A,D'Alfonso A,Carbone MG,Carta G,Festuccia C,Gaudio E,Colapietro A,Sferra R

PA1-075 was used in Immunohistochemistry on paraffin embedded tissues to evaluate the Advanced Glycation End (AGE) products, ERK1/2 and transforming growth factor (TGF)-β/Smad pathway expression in muscularis propria in women with POP compared with control patients.

Sat Sep 01 00:00:00 EDT 2018

Amyloid-beta accumulation, neurogenesis, behavior, and the age of rats.

Behavioral neuroscience

Church RM,Miller MC,Freestone D,Chiu C,Osgood DP,Machan JT,Messier AA,Johanson CE,Silverberg GD

PA1-075 was used in immunohistochemistry - frozen section to study the relationship between brain biochemistry and behavior that are age-related

Fri Aug 01 00:00:00 EDT 2014

Blockage of receptor for advanced glycation end products prevents development of cardiac dysfunction in db/db type 2 diabetic mice.

European journal of heart failure

Nielsen JM,Kristiansen SB,Nørregaard R,Andersen CL,Denner L,Nielsen TT,Flyvbjerg A,Bøtker HE

PA1-075 was used in western blot to investigate the role of receptor for advanced glycation end products during development of cardiac dysfunction in db/db type 2 diabetic mice

Wed Jul 01 00:00:00 EDT 2009

Blockage of receptor for advanced glycation end products prevents development of cardiac dysfunction in db/db type 2 diabetic mice.

European journal of heart failure

Nielsen JM,Kristiansen SB,Nørregaard R,Andersen CL,Denner L,Nielsen TT,Flyvbjerg A,Bøtker HE

PA1-075 was used in western blot to investigate the role of receptor for advanced glycation end products during development of cardiac dysfunction in db/db type 2 diabetic mice

Wed Jul 01 00:00:00 EDT 2009

Vitamin E increases S100B-mediated microglial activation in an S100B-overexpressing mouse model of pathological aging.

Glia

Bialowas-McGoey LA,Lesicka A,Whitaker-Azmitia PM

PA1-075 was used in immunohistochemistry to investigate the effect of vitamin E on S100B-mediated microglial activation

Mon Dec 01 00:00:00 EST 2008

Weight increase is associated with skeletal muscle immunostaining for advanced glycation end products, receptor for advanced glycation end products, and oxidation injury.

Rejuvenation research

de la Maza MP,Uribarri J,Olivares D,Hirsch S,Leiva L,Barrera G,Bunout D

PA1-075 was used in immunohistochemistry to investigate the influence of weight increasement on structure and gene expression in skeletal muscles

Mon Dec 01 00:00:00 EST 2008

High-mobility group box 1 protein is an inflammatory mediator in necrotizing enterocolitis: protective effect of the macrophage deactivator semapimod.

American journal of physiology. Gastrointestinal and liver physiology

Zamora R,Grishin A,Wong C,Boyle P,Wang J,Hackam D,Upperman JS,Tracey KJ,Ford HR

PA1-075 was used in western blot to study the role of high-mobility group box 1 protein in necrotizing enterocolitis

Sat Oct 01 00:00:00 EDT 2005

Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture.

Nature biotechnology

Durr E,Yu J,Krasinska KM,Carver LA,Yates JR,Testa JE,Oh P,Schnitzer JE

PA1-075 was used in western blot to study the proteome of the rat lung microvascular endothelial cell surface in vivo and in cultured rat lung ECs

Sun Aug 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: advanced glycation end-products receptor; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor variant 2; advanced glycosylation end product-specific receptor variant 3; advanced glycosylation end product-specific receptor variant 4; advanced glycosylation end product-specific receptor variant 5; RAGE-4 ORF3; receptor for advanced glycation end-products variant 20; Receptor for advanced glycosylation end products; sRAGE

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Gene Aliases: Ager; RAGE

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UniProt ID: (Mouse) Q62151

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Entrez Gene ID: (Rat) 81722, (Mouse) 11596

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Function(s)

protein binding identical protein binding S100 protein binding advanced glycation end-product receptor activity high mobility group box 1 binding cell adhesion molecule immunoglobulin superfamily cell adhesion molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAGE Polyclonal Antibody

RAGE Antibody (PA1-075) in IHC (P)

Product Details

PA1-075

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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