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Product Details
CF506821
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1F2
Immunogen
Full length human recombinant protein of human RAPGEF1 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1
Gene Aliases: C3G; GRF2; RAPGEF1
UniProt ID: (Human) Q13905
Entrez Gene ID: (Human) 2889
activation of MAPKK activity
blood vessel development
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of neuron projection development
single organismal cell-cell adhesion
Rap protein signal transduction
nerve growth factor signaling pathway
positive regulation of GTPase activity
negative regulation of Ras protein signal transduction
platelet-derived growth factor receptor signaling pathway
negative regulation of protein kinase B signaling
establishment of endothelial barrier
negative regulation of ERK1 and ERK2 cascade
cellular response to cAMP
negative regulation of canonical Wnt signaling pathway
regulation of cell junction assembly
cellular response to nerve growth factor stimulus
negative regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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