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RCAS1 Antibody (PA5-102523) in ICC/IF
Immunofluorescent analysis of RCAS1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and RCAS1 polyclonal antibody (Product # PA5-102523) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102523
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EBAG9(Accession O00559), corresponding to amino acid residues Q175-S213.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851925
Product Specific Information
Antibody detects endogenous levels of total EBAG9.
Target Information
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG9; cancer associated surface antigen; cancer associated surface antigen RCAS1; Cancer-associated surface antigen RCAS1; estrogen receptor binding fragment associated gene 9; Estrogen receptor-binding fragment-associated gene 9 protein; HGNC:3123; receptor binding cancer antigen expressed on SiSo cells; Receptor-binding cancer antigen expressed on SiSo cells
Gene Aliases: AI835379; EB9; EBAG9; PDAF; RCAS1
UniProt ID: (Human) O00559, (Mouse) Q9D0V7, (Rat) Q5PQP2
Entrez Gene ID: (Human) 9166, (Mouse) 55960, (Rat) 299864
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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