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RCAS1 Antibody (MA5-29229) in WB
Western Blot using RCAS1 Recombinant Rabbit Monoclonal Antibody (19) (Product # MA5-29229) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa. (We are unsure of the identity of these extra bands).
Product Details
MA5-29229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
019
Immunogen
Recombinant Human RCAS1/EBAG9 Protein (Arg28-Ser213)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785130
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human RCAS1/EBAG9.
Target Information
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG9; cancer associated surface antigen; cancer associated surface antigen RCAS1; Cancer-associated surface antigen RCAS1; estrogen receptor binding fragment associated gene 9; Estrogen receptor-binding fragment-associated gene 9 protein; HGNC:3123; receptor binding cancer antigen expressed on SiSo cells; Receptor-binding cancer antigen expressed on SiSo cells
Gene Aliases: EB9; EBAG9; PDAF; RCAS1
UniProt ID: (Human) O00559
Entrez Gene ID: (Human) 9166
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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