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Invitrogen
RELM alpha Monoclonal Antibody (DS8RELM), PE, eBioscience™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
RELM alpha Antibody (12-5441-82) in Flow
C57BL/6 mouse resident peritoneal exudate cells were surface stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-4801-82). The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and intracellularly stained with either 0.25 µg of Rat IgG1 kappa Isotype Control, PE (Product # 12-4301-82) (left) or 0.25 µg of RELM alpha Monoclonal Antibody, PE (Product # 12-5441-82)(right). All cells were used for analysis.
Product Details
12-5441-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DS8RELM
Immunogen
Recombinant mouse RELM alpha protein
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762682
Product Specific Information
Description: This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1. This antibody was shown not to cross-react with mouse RELM gamma.
Applications Reported: This DS8RELM antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DS8RELM antibody has been tested by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; Hypoxia-induced mitogenic factor; Parasite-induced macrophage novel gene 1 protein; RELM-beta; RELMalpha; RELMb; Resistin-like alpha
Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; Pmng1; RELM-alpha; RELMa; RELMalpha; Retnla; Xcp2
UniProt ID: (Mouse) Q9EP95
Entrez Gene ID: (Mouse) 57262
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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